BioXCell熱銷產(chǎn)品--RecombiMAb anti-mouse Ly6G
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產(chǎn)品描述：
1A8-CP129單克隆抗體是原始1A8單克隆抗體的重組嵌合型抗體。可變結(jié)構(gòu)域序列與原始1A8相同，但是恒定區(qū)序列已經(jīng)從大鼠IgG2a變?yōu)樾∈驣gG2a。1A8-CP129單克隆抗體像原始克隆號(hào)的大鼠IgG2a抗體一樣，不包含F(xiàn)c突變。
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1A8-CP129單克隆抗體與小鼠Ly6G反應(yīng)。Ly6G分子量為21-25kDa，是GPI錨定的細(xì)胞表面蛋白Ly-6超家族的成員，在細(xì)胞信號(hào)傳導(dǎo)和細(xì)胞粘附中發(fā)揮作用。Ly6G在發(fā)育過(guò)程中由骨髓譜系中的細(xì)胞（包括單核細(xì)胞、巨噬細(xì)胞、粒細(xì)胞和嗜中性粒細(xì)胞）差異表達(dá)。單核細(xì)胞通常在發(fā)育過(guò)程中瞬時(shí)表達(dá)Ly6G，而成熟的粒細(xì)胞和外周嗜中性粒細(xì)胞持續(xù)表達(dá)，使Ly6G成為這些細(xì)胞群體的表面標(biāo)志物。與BioXcell RB6-8C5單克隆抗體不同，1A8-CP129單克隆抗體與小鼠Ly6G特異性反應(yīng)，而與Ly6C沒(méi)有交叉反應(yīng)性的報(bào)道。
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產(chǎn)品詳情：
產(chǎn)品名稱 | RecombiMAb anti-mouse Ly6G |
產(chǎn)品貨號(hào) | CP129 |
產(chǎn)品規(guī)格 | 1/5/25/50/100mg |
反應(yīng)種屬 | Mouse |
克隆號(hào) | 1A8-CP129 |
同種型 | Mouse IgG2a(switched from rat IgG2a) |
免疫原 | EL4J cells transfected with Ly6G |
實(shí)驗(yàn)應(yīng)用 | in vivo neutrophil depletion* in vivo MDSC depletion* Immunofluorescence* Immunohistochemistry (paraffin)* Immunohistochemistry (frozen)* Flow cytometry* *Reported for the original rat IgG2a 1A8 antibody |
產(chǎn)品形式 | PBS, pH 7.0,Contains no stabilizers or preservatives |
純度 | >95%, Determined by SDS-PAGE |
聚合 | <5%, Determined by SEC |
無(wú)菌處理 | 0.2 μm filtration |
純化方式 | Protein G |
分子量 | 150 kDa |
小鼠病原檢測(cè) | Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative |
保存條件 | 抗體原液保存在4°C，不能冷凍保存。 |
推薦同型對(duì)照 | InVivoPlus mouse IgG2a isotype control, unknown specificity（貨號(hào)BP0085） |
推薦抗體稀釋液 | InVivoPure pH 7.0 Dilution Buffer（貨號(hào)IP0070） |
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該產(chǎn)品自上市已被多篇SCI文獻(xiàn)引用，品質(zhì)有保證，以下是部分已發(fā)表的文獻(xiàn)引用：
應(yīng)用 | 文章 |
體內(nèi)中性粒細(xì)胞耗竭 (in vivo?neutrophil depletion) | 1.?Davis, R. W. t., et al. (2018). 'Luminol Chemiluminescence Reports Photodynamic? Therapy-Generated Neutrophil Activity In Vivo and Serves as a Biomarker of Therapeutic? Efficacy' Photochem Photobiol . 2. Moynihan, K. D., et al. (2016). 'Eradication of large established tumors in mice by? combination immunotherapy that engages innate and adaptive immune responses' Nat Med.? doi : 10.1038/nm.4200. 3.?Conde, P., et al. (2015). 'DC-SIGN(+) Macrophages Control the Induction of Transplantation? Tolerance' Immunity 42(6): 1143-1158. 4.?Griseri, T., et al. (2015). 'Granulocyte Macrophage Colony-Stimulating Factor-Activated? Eosinophils Promote Interleukin-23 Driven Chronic Colitis' Immunity 43(1): 187-199. 5. Yamada, D. H., et al. (2015). 'Suppression of Fcgamma-receptor-mediated antibody? effector function during persistent viral infection' Immunity 42(2): 379-390.? |
體內(nèi)中性粒細(xì)胞耗竭、流式細(xì)胞術(shù)、 免疫組化石蠟切片(in vivo neutrophil? depletion, Flow Cytometry,? Immunohistochemistry (paraffin)) | Coffelt, S. B., et al. (2015). 'IL-17-producing gammadelta T cells and neutrophils conspire? to promote breast cancer metastasis' Nature 522(7556): 345-348. |
體內(nèi)中性粒細(xì)胞耗竭、流式細(xì)胞術(shù)、 免疫組化石蠟切片、免疫組化冰凍切片 (in vivo?neutrophil depletion, Flow? Cytometry, Immunohistochemistry? (paraffin), Immunohistochemistry?(frozen) | Finisguerra, V., et al. (2015). 'MET is required for the recruitment of anti-tumoural? neutrophils' Nature 522(7556): 349-353. |
體內(nèi)中性粒細(xì)胞耗竭、流式細(xì)胞術(shù) (in vivo neutrophil depletion,? Flow Cytometry) | 1.Moser, E. K., et al. (2014). 'Late engagement of CD86 after influenza virus? clearance promotes recovery in a FoxP3+ regulatory T cell dependent manner'? PLoS Pathog 10(8): e1004315. 2. Chen, K. W., et al. (2014). 'The neutrophil NLRC4 inflammasome selectively? promotes IL-1beta maturation without pyroptosis during acute Salmonella? challenge' Cell Rep 8(2): 570-582. 3. Garraud, K., et al. (2012). 'Differential role of the interleukin-17 axis and? neutrophils in resolution of inhalational anthrax' Infect Immun 80(1): 131-142. |
體內(nèi)骨髓來(lái)源抑制細(xì)胞耗竭 (in vivo?MDSC depletion) | Deng, L., et al. (2014). 'Irradiation and anti-PD-L1 treatment synergistically? promote antitumor immunity in mice' J Clin Invest 124(2): 687-695. |
體內(nèi)中性粒細(xì)胞耗竭、 免疫熒光(in vivo?neutrophil? depletion, Immunofluorescence) | Edelson, B. T., et al. (2011). 'CD8alpha(+) dendritic cells are an obligate? cellular entry point for productive infection by Listeria monocytogenes'? Immunity 35(2): 236-248. |
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