產(chǎn)品貨號

CP002

產(chǎn)品規(guī)格

1mg

反應(yīng)種屬

Mouse

克隆號

RMP1-14-CP002

同種型

Mouse IgG1(switched from rat IgG2a)

免疫原

Syrian Hamster BKH cells transfected with mouse PD-1 cDNA

實(shí)驗(yàn)應(yīng)用

in vivo blocking of PD-1/PD-L signaling*

*Reported for the original rat IgG2a RMP1-14 antibody

產(chǎn)品形式

PBS, pH 7.0,Contains no stabilizers or preservatives

純度

>95%, Determined by SDS-PAGE

聚合

<5%, Determined by SEC

無菌處理

0.2 μm filtration

純化方式

Protein G

分子量

150 kDa

小鼠病原檢測

Ectromelia/Mousepox Virus: Negative

Hantavirus: Negative

K Virus: Negative

Lactate Dehydrogenase-Elevating Virus: Negative

Lymphocytic Choriomeningitis virus: Negative

Mouse Adenovirus: Negative

Mouse Cytomegalovirus: Negative

Mouse Hepatitis Virus: Negative

Mouse Minute Virus: Negative

Mouse Norovirus: Negative

Mouse Parvovirus: Negative

Mouse Rotavirus: Negative

Mycoplasma Pulmonis: Negative

Pneumonia Virus of Mice: Negative

Polyoma Virus: Negative

Reovirus Screen: Negative

Sendai Virus: Negative

Theiler’s Murine Encephalomyelitis: Negative

保存條件

抗體原液保存在4°C，不能冷凍保存。

推薦抗體稀釋液

InVivoPure pH 7.0 Dilution Buffer（貨號IP0070）

體內(nèi)PD-1/PD-L信號阻斷

(in vivo blocking of PD-1/PD-L?

signaling)

1.?Triplett, T. A., et al. (2018). 'Reversal of indoleamine 2,3-dioxygenase-mediated cancer?

immune suppression by systemic kynurenine depletion with a therapeutic enzyme' Nat?

Biotechnol 36(8): 758-764.

2.?Grasselly, C., et al. (2018). 'The Antitumor Activity of Combinations of Cytotoxic?

Chemotherapy and Immune Checkpoint Inhibitors Is Model-Dependent' Front Immunol 9: 2100.

3.?Moynihan, K. D., et al. (2016). 'Eradication of large established tumors in mice by?

combination immunotherapy that engages innate and adaptive immune responses' Nat?

Med. doi : 10.1038/nm.4200.

4.?Ngiow, S. F., et al. (2015). 'A Threshold Level of Intratumor CD8+ T-cell PD1 Expression?

Dictates Therapeutic Response to Anti-PD1' Cancer Res 75(18): 3800-3811. ?

5.?Evans, E. E., et al. (2015). 'Antibody Blockade of Semaphorin 4D Promotes Immune?

Infiltration into Tumor and Enhances Response to Other Immunomodulatory Therapies'?

Cancer Immunol Res 3(6): 689-701.

6.?Zelenay, S., et al. (2015). 'Cyclooxygenase-Dependent Tumor Growth through Evasion?

of Immunity' Cell 162(6): 1257-1270.

7.?Zander, R. A., et al. (2015). 'PD-1 Co-inhibitory and OX40 Co-stimulatory Crosstalk?

Regulates Helper T Cell Differentiation and Anti-Plasmodium Humoral Immunity' Cell?

Host Microbe 17(5): 628-641.