AbD Serotec公司生產(chǎn)的Rat anti Brdu, clone BU1/75(ICR1), 能夠識(shí)別游離的以及和單鏈DNA結(jié)合的Brdu, 是目前文獻(xiàn)引用最多的anti-Brdu抗體, 同時(shí)AbD Serotec是該抗體唯一的商品化生產(chǎn)商。
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Ki-67是一種增殖細(xì)胞相關(guān)的核抗原, 不表達(dá)于靜止期的細(xì)胞, Ki-67的反義寡核苷酸能夠抑制細(xì)胞的增殖, 揭示Ki-67蛋白對(duì)細(xì)胞增殖是絕對(duì)必需的。
福爾馬林固定, 石蠟包埋的小鼠結(jié)腸組織切片, ? ? ? ? ?MCF-7細(xì)胞的anti Ki-67免疫熒光染色結(jié)果
Rat anti Brdu(MCA2060)染色結(jié)果 ? ? ? ? ? ? ? ? ? ? ? ? ? ? (HCA006, 綠色), Phalloidin(紅色)和Hoechst
? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?(藍(lán)色)復(fù)染。
AbD Serotec公司提供的最受歡迎的細(xì)胞增殖標(biāo)志物抗體
| ?產(chǎn)品編號(hào) | ?抗體類型 | ?特異性 | ?克隆號(hào) | ?宿主 | ?適用種屬 | ?形式 | ?規(guī)格 | ?應(yīng)用 |
| ?MCA2060 | ?mAb | ?BrdU | ?BU1/75
?(ICR1) | ?Rat |
| ?Purified | ?0.2 mg | ?F*, IF, P* |
| ?MCA2060GA | ?mAb | ?BrdU | ?BU1/75
?(ICR1) | ?Rat |
| ?Purified | ?0.1 mg | ?F*, IF, P* |
| ?MCA2060T | ?mAb | ?BrdU | ?BU1/75 ?(ICR1) | ?Rat |
| ?Purified | ?20 μg | ?F*, IF, P* |
| MCA2060A488 | mAb | BrdU | BU1/75 (ICR1) | Rat |
| ALEXA FLUOR? 488 | 100 TESTS/1ml | F* |
| MCA2060B | mAb | BrdU | BU1/75 (ICR1) | Rat |
| Biotin | 0.1 mg | F* |
| MCA2060F | mAb | BrdU | BU1/75 (ICR1) | Rat |
| FITC | 0.1 mg | F* |
| MCA2060FT | mAb | BrdU | BU1/75 (ICR1) | Rat |
| FITC | 0.25 mg | F* |
| MCA2060P | mAb | BrdU | BU1/75 (ICR1) | Rat |
| HRP | 0.5 mg | E, P* |
| MCA2060PA | mAb | BrdU | BU1/75 (ICR1) | Rat |
| HRP | 0.1 mg | E, P* |
| MCA2483 | mAb | BrdU | Bu20a | Mouse |
| Purified | 0.2 mg | C, F*, P* |
| MCA2483FA | mAb | BrdU | Bu20a | Mouse |
| FITC | 50 μg | F* |
| MCA2483T | mAb | BrdU | Bu20a | Mouse |
| Purified | 20 μg | C, F*, P* |
| HCA006 | mAb | Ki-67 | 2531 (Fab) | HuCAL? | Human, Bovine, Dog | Purified | 0.1 mg | C, E, IF, P*,WB |
| HCA053 | mAb | Ki-67 | 2531 | HuCAL? | Human, Bovine, Dog | Purified | 0.1 mg | C, E, P*, WB |
| MAb | mAb | Ki-67 | 2531 | HuCAL? | Human, Bovine,Dog | Purified | 50 μg | C, E, P*, WB |
| HCA117 | mAb | Ki-67 | 2815 | HuCAL? | Human | Purified | 0.1 mg | E, P* |
| HCA117A | mAb | Ki-67 | 2815 | HuCAL? | Human | Purified | 50 μg | E, P* |
| MCA289 | mAb | Ki-67 | Ki67 | Mouse | Human | Supernatant | 2 ml | C*, F*, IF |
| MCA289T | mAb | Ki-67 | Ki67 | Mouse | Human | Supernatant | 0.2 ml | C*, F*, IF |
| MCA1859 | mAb | MCM2 | CRCT2.1 | Mouse | Human | Purified | 0.1 mg | C, P*, WB |
| AHP1419 | pAb | PCNA |
| Rabbit | Broad | Purified | 50 μg | E, P*, WB |
| MCA1558 | mAb | PCNA | PC10 | Mouse | Broad | Purified | 0.1 mg | C, F*, IP, P,WB |
| MCA1558F | mAb | PCNA | PC10 | Mouse | Broad | FITC | 0.1 mg | F* |
| MCA1558H | mAb | PCNA | PC10 | Mouse | Broad | Purified | ?6 ml | C, P |
| MCA3328Z | mAb | PCNA | 1G7 | Mouse | Human | Azide Free | 0.1 mg | IF, WB |
AbD serotec公司Brdu抗體參考文獻(xiàn)
1.???? Vanderlaan, M. & Thomas, C.B. (1985) Characterization of monoclonal antibodies to bromodeoxyuridine.Cytometry. 6: 501-505.
2.???? Ghiringelli, F.?et al.?(2005) Tumor cells convert immature myeloid dendritic cells into TGF-beta-secreting cells inducing CD4+CD25+ regulatory T cell proliferation.J. Exp. Med. 202: 919-929.
3.???? Dolbeare, F. (1995) Bromodeoxyuridine: a diagnostic tool in biology and medicine, Part I: Historical perspectives, histochemical methods and cell kinetics.Histochem. J. 27: 339-369.
4.???? Micheli, L.?et al.?(2010) PC4/Tis7/IFRD1 stimulates skeletal muscle regeneration and is involved in myoblast differentiation as a regulator of MyoD and NF-kB.J Biol Chem. Dec 14. [Epub ahead of print]
5.???? Das, G.?et al.?(2009) Cyclin D1 fine-tunes the neurogenic output of embryonic retinal progenitor cells.Neural Dev. 4: 15.
6.???? Nakhai, H.?et al.?(2008) Conditional ablation of Notch signaling in pancreatic development.Development. 135: 2757-65.
7.???? Ghai, K.?et al.?(2010) Notch signaling influences neuroprotective and proliferative properties of mature Müller glia.?
J Neurosci. 30: 3101-12.
8.???? Kim, T.H.?et al.?(2011) Genetic evidence that intestinal notch functions vary regionally and operate through a common mechanism of math1 repression.J Biol Chem. Jan 31. [Epub ahead of print]
9.???? Sabo, J.K.?et al.?(2011) Remyelination is altered by bone morphogenic protein signaling in demyelinated lesions.
J Neurosci. 31: 4504-10.
10.?? Bourgo, R.J.?et al.?(2011) RB deletion disrupts coordination between DNA replication licensing and mitotic entry?in vivo.Mol Biol Cell. 22: 931-9.
11.?? Bugler, B.?et al.?(2010) Unscheduled expression of CDC25B in S-phase leads to replicative stress and DNA damage.
Mol Cancer. 9: 29.
12.?? Caronia, G.?et al.?(2010) Bone morphogenetic protein signaling in the developing telencephalon controls formation of the hippocampal dentate gyrus and modifies fear-related behavior.J Neurosci. 30: 6291-301.
13.?? Xu, Q.?et al.?(2010) Sonic hedgehog signaling confers ventral telencephalic progenitors with distinct cortical interneuron fates.Neuron. 65: 328-40.
14.?? Puverel, S.?et al.?(2011) RanBPM is essential for mouse spermatogenesis and oogenesis.Development. 138: 2511-21.
15.?? Amador-Arjona, A.?et al.?(2011) Primary cilia regulate proliferation of amplifying progenitors in adult hippocampus: implications for learning and memory.J Neurosci. 31: 9933-44.
16.?? Chojnacki, A.?et al.?(2011) PDGFR{alpha} Expression Distinguishes GFAP-Expressing Neural Stem Cells from PDGF-Responsive Neural Precursors in the Adult Periventricular Area.J Neurosci. 31: 9503-12.
17.?? Kroehne, V.?et al.?(2011) Regeneration of the adult zebrafish brain from neurogenic radial glia-type progenitors.
Development. 138: 4831-41.
18.?? Bonzo, J.A.?et al.?(2012) Suppression of Hepatocyte Proliferation by Hepatocyte Nuclear Factor 4a in Adult Mice.
J Biol Chem. Jan 12. [Epub ahead of print]
19.?? Liu, M.T.?et al.?(2009) 5-HT4 receptor-mediated neuroprotection and neurogenesis in the enteric nervous system of adult mice.J Neurosci. 29: 9683-99.
20.?? Lundgren, O.?et al.?(2011) Intestinal epithelial stem/progenitor cells are controlled by mucosal afferent nerves.
PLoS One. 6: e16295.
21.?? Knopf, F.?et al.?(2011) Bone regenerates via dedifferentiation of osteoblasts in the zebrafish fin.Dev Cell. 20: 713-24.
22.?? Contet, C.?et al.?(2013) μ-Opioid receptors mediate the effects of chronic ethanol binge drinking on the hippocampal neurogenic niche.Addict Biol. Mar 6. [Epub ahead of print]
23.?? Grotek, B.?et al.?(2013) Notch signaling coordinates cellular proliferation with differentiation during zebrafish fin regeneration.Development. 140: 1412-23.
24.?? Lee, N.?et al.?(2013) Ciliary neurotrophic factor receptor regulation of adult forebrain neurogenesis.
J Neurosci. 33: 1241-58.
25.?? Wang, W.?et al.?(2013) Extracellular signal-regulated kinase 5 (ERK5) mediates prolactin-stimulated adult neurogenesis in the subventricular zone and olfactory bulb.J Biol Chem. 288: 2623-31
26.?? Zemans, R.L.?et al.?(2013) Role of ?-catenin-Regulated CCN Matricellular Proteins in Epithelial Repair After Inflammatory Lung Injury.Am J Physiol Lung Cell Mol Physiol. Jan 11. [Epub ahead of print]
27.?? Zimmerman, K.M.?et al.?(2013) Diminished origin licensing capacity specifically sensitises tumour cells to replication stress.Mol Cancer Res. Jan 30. [Epub ahead of print]
28.?? Maden, M.?et al.?(2013) Proliferation zones in the axolotl brain and regeneration of the telencephalon.Neural Dev. 8: 1.?