血管內(nèi)皮生長因子(VEGF)最初被稱為血管通透性因子(VPF),是一個刺激血管發(fā)生和血管新生的蛋白質(zhì)家族。VEGF家族包括五個成員:VEGF-A、VEGF-B、VEGF-C、VEGF-D和PGF(胎盤生長因子)。作為第一個被發(fā)現(xiàn)的VEGF家族成員,VEGF-A常被簡稱為VEGF。VEGF-A基因由8個外顯子組成,選擇性剪接產(chǎn)生10種以上亞型,包括VEGF 121、VEGF 145、VEGF 165、VEGF 183、VEGF 189和VEGF 206等。VEGF165和VEGF121是在大多數(shù)細(xì)胞和組織中表達(dá)的主要亞型。VEGF165是一種45KDa同源二聚體糖蛋白,對肝素具有中等親和力。相反,VEGF121不與肝素結(jié)合。VEGF特異性結(jié)合酪氨酸激酶受體VEGFR-1(Flt-1)和VEGFR-2(Flk-1),激活下游信號通路,導(dǎo)致內(nèi)皮細(xì)胞存活、增殖、遷移和血管通透性。VEGF通過增強血管通透性或招募內(nèi)皮祖細(xì)胞到新生血管形成部位遠(yuǎn)處位點,在病理性血管生成中起著核心作用。隨著對VEGF促進病理性血管生成和腫瘤轉(zhuǎn)移的深入了解,在多種臨床模型中,阻斷VEGF/VEGFR軸抑制血管生成和腫瘤生長已成為合理的研究方向。
ENZO VEGF ELISA kit是一種靈敏的比色酶免疫測定試劑盒,可在2.5小時內(nèi)得到檢測結(jié)果。在450 nm處讀取吸光度。
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●?高靈敏度,可檢測低至4.712 pg/ml的VEGF
●?高特異性,與VEGF 165的交叉反應(yīng)為100%,與包括VEGF 121在內(nèi)的相關(guān)分子幾乎沒有交叉反應(yīng)
●?高通量,可檢測多達(dá)38個樣本(帶復(fù)孔),僅需2.5h即可獲得結(jié)果
●?彩色編碼試劑有助于減少錯誤并提高效率
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產(chǎn)品信息
產(chǎn)品貨號 | ENZ-KIT156-0001 |
產(chǎn)品名稱 | VEGF (human), ELISA kit(人血管內(nèi)皮生長因子ELISA試劑盒) |
規(guī)格 | 96 wells |
其他名稱 | Vascular endothelial growth factor |
靈敏度 | 4.712 pg/ml |
檢測范圍 | 8-2,000 pg/ml |
檢測時間 | 2.5 hours |
樣本種屬 | Human |
樣本類型 | human serum, plasma, and culture supernatants |
保存溫度 | +4°C |
試劑盒組分 | Microtiter Plate, Conjugate, Detector Antibody, Assay buffer, Antibody Diluent, Wash Buffer Concentrate, Standard, TMB Substrate, Stop Solution |
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部分產(chǎn)品文獻(xiàn)引用
1. Negative Modulation of the Angiogenic Cascade Induced by Allosteric Kinesin Eg5 Inhibitors in a Gastric Adenocarcinoma In Vitro Model: A. Ricci, et al.; Molecules 27, 957 (2022)?
2. Attenuation of high glucose-induced damage in RPE cells through p38 MAPK signaling pathway inhibition: G. Maugeri, et al.; Front. Pharmacol. 12, 684680 (2021), Application(s): ELISA using culture supernatants?
3. Mifepristone as a potential therapy to reduce angiogenesis and p-glycoprotein associated with glioblastoma resistance to temozolomide: M. Llaguno-Munive, et al.; Front. Oncol. 10, 581814 (2020)?
4. The combination of sulforaphane and Fernblock? XP improves individual beneficial effects in normal and neoplastic human skin cell lines: S. Serini, et al.; Nutrients 12, 1608 (2020), Application(s): ELISA using culture supernatants
5. Aflibercept regulates retinal inflammation elicited by high glucose via the PlGF/ERK pathway: F. Lazzara, et al.; Biochem. Pharmacol. 168, 341 (2019), Application(s): ELISA using culture supernatants
6. Mechanism of the anti-angiogenic effect of avemar on tumor cells: N.G. Imir, et al.; Oncol. Lett. 15, 2673 (2018), Application(s): ELISA using culture supernatants?
7. The Role of Human Umbilical Vein Endothelial Cells in Osteogenic Differentiation of Dental Follicle-Derived Stem Cells in In Vitro Co-cultures: J.S. Bok, et al.; Int. J. Med. Sci. 15, 1160 (2018)
8. The effect of etanercept on vascular endothelial growth factor production by cutaneous mesenchymal stem cells from patients with psoriasis: A. Campanati, et al.; J. Int. Med. Res. 44, 6 (2016), Application(s): VEGF quantification, human MSC cells?
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