DNA損傷酶聯(lián)免疫檢測試劑盒DNA damage ELISA kit—Enzo Life Sciences熱銷產(chǎn)品
——用于腫瘤、細(xì)胞凋亡和氧化應(yīng)激研究中DNA損傷的快速檢測
細(xì)胞暴露于氧化和環(huán)境應(yīng)激經(jīng)常導(dǎo)致基因組DNA的分解或氧化，評(píng)價(jià)基因組DNA完整性或評(píng)估氧化DNA存在的測定法經(jīng)常用作驗(yàn)證凋亡或DNA損傷開始的手段。8-羥基-2 '-脫氧鳥苷(8-OHdG)是一種修飾的核苷堿基，是氧化DNA損傷和氧化應(yīng)激的常用生物標(biāo)志物。尿液中的8-OHdG及其類似物8-羥基鳥苷和8-羥基鳥嘌呤與許多退行性疾病有關(guān)。在許多疾病中，包括膀胱癌和前列腺癌，囊性纖維化，特應(yīng)性皮炎和類風(fēng)濕性關(guān)節(jié)炎中，已經(jīng)研究了ROS的相關(guān)性和使用8-OHdG作為氧化應(yīng)激的生物標(biāo)志物。帕金森氏病、阿爾茨海默氏病和亨廷頓氏病是神經(jīng)退行性疾病，被認(rèn)為是由具有這些疾病遺傳傾向的人接觸神經(jīng)毒素引起的。氧化應(yīng)激與這些疾病的發(fā)病機(jī)制有關(guān)，在多種神經(jīng)系統(tǒng)疾病中檢測到DNA損傷水平升高。
Enzo Life Sciences的DNA損傷酶聯(lián)免疫檢測試劑盒是一種快速靈敏的競爭法免疫檢測試劑盒，可用于檢測和定量尿液、血清和唾液樣品中的8-羥基-2 '-脫氧鳥苷(8- OHdG)。
產(chǎn)品特點(diǎn)
● 高靈敏度，可檢測低至1 ng/ml 8-OHdG
●?在多類型樣本中驗(yàn)證并獲得可靠結(jié)果
●?優(yōu)于半定量Western blot分析的全定量檢測
●?無邊緣效應(yīng)，每個(gè)試劑盒可檢測更多樣本
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產(chǎn)品詳情
產(chǎn)品貨號(hào) | ADI-EKS-350 |
產(chǎn)品名稱 | DNA damage ELISA kit（DNA損傷ELISA試劑盒） |
規(guī)格 | 96 wells |
靈敏度 | 0.59 ng/ml |
檢測范圍 | 0.94 - 60 ng/ml |
檢測時(shí)間 | <2.5 hours |
適用樣本類型 | 血清，唾液，尿液（有文獻(xiàn)報(bào)道血漿、精液、細(xì)胞裂解物、培養(yǎng)上清液和DNA提取物） |
適用種屬 | 不限物種 |
交叉反應(yīng) | The DNA Damage ELISA also detects 8-hydroxyguanosine (product of oxidative RNA damage)? and 8-hydroxyguanine (product of oxidative DNA damage by hydroxyl radicals) |
試劑盒組分 | Microtiter plate, Standard, Sample diluent, Wash buffer concentrate, Antibody, Antibody diluent,? Conjugate, Conjugate diluent, TMB Substrate, Stop solution 2 |
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標(biāo)準(zhǔn)曲線示例
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部分產(chǎn)品文獻(xiàn)引用
1. The Dietary Fermentable Fiber Inulin Alters the Intestinal Microbiome and Improves Chronic?Kidney Disease Mineral-Bone Disorder in a Rat Model of CKD: A. Biruete, et al.; bioRxiv (2023)?
2. Octanoic Acid - An Insecticidal Metabolite of Conidiobolus coronatus (Entomopthorales) That Affects Two Majors Antifungal Protection Systems in Galleria mellonella (Lepidoptera): Cuticular Lipids and Hemocyte: A. Kaczmarek, et al.; Int. J. Mol. Sci. 23, 5204 (2022)
3. Wasp Venom Ameliorates Scopolamine-Induced Learning and Memory Impairment in Mice: J.H. Chae, et al.; Toxins 14, 256 (2022)
4. Arbutin protects brain against middle cerebral artery occlusion-reperfusion (MCAo/R) injury: M. Kumar, et al.; Biochem. Biophys. Res. Commun. 577, 52 (2021)
5. Berberine ameliorates renal injury in a rat model of D-galactose-induced aging through a PTEN/Akt-dependent mechanism: H.E. El-Horany, et al.; Arch. Physiol. Biochem. 126, 157 (2020), Application(s): Rat blood serum
6. Evaluation of Effectiveness of Ultraviolet Emitting Lamps on the Cutaneous Production of Vitamin D3: Relationship of the Lamps Vitamin D3 Producing Potential to the Production of 8-Hydroxy-2'-Deoxyguanosine and Nitric Oxide: A. Shirvani, et al.; Anticancer Res. 40, 565 (2020)
7. In Vivo Anti-Inflammatory Potential of Viscozyme?-Treated Jujube Fruit: Y. Kim, et al.; Foods 9, 1033 (2020), Application(s): Mouse Liver tissue homogenate
8. Inhibitory Effect of Steamed Soybean Wastewater Against DSS-Induced Intestinal Inflammation in Mice: S. Jeong, et al.; Foods 9, 954 (2020), Application(s): Quantification of 8-OHdG level in mouse plasma.
9. N-acetylcysteine (NAC), an anti-oxidant, does not improve bone mechanical properties in a rat model of progressive chronic kidney disease-mineral bone disorder: M.R. Allen, et al.; PLoS One 15, e0230379 (2020)
10. Neuroprotective Effects of Euonymus alatus Extract on Scopolamine-Induced Memory Deficits in Mice: Y. Woo, et al.; Antioxidants (Basel) 9, 449 (2020), Application(s): Serum
11. Antioxidative effects of olive polyphenols compared to vitamin E in piglets fed a diet rich in N-3 PUFA: J. Leskovec, et al.; Animals (Basel) 9, 161 (2019), Application(s): ELISA using pig serum
12. MicroRNA-204-3p Attenuates High Glucose-Induced MPC5 Podocytes Apoptosis by Targeting Braykinin B2 Receptor: X. Han, et al.; Exp. Clin. Endocrinol. Diabetes 127, 387 (2019), Application(s): Mouse MPC5 podocyte lysates
13. Oxidative stress and mitochondrial responses to stress exposure suggest that king penguins are naturally equipped to resist stress: A. Stier, et al.; Sci. Rep. 9, 8545 (2019), Application(s): Penguin plasma and red blood cell lysate
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