ENZO熱銷產(chǎn)品——TOTAL-NUCLEAR-ID? green/red nucleolar/nuclear detection kit核仁／細(xì)胞核檢測試劑盒（綠色／紅色）
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ENZO的TOTAL-NUCLEAR-ID? green/red nucleolar/nuclear detection kit包含兩種專有染料，適用于同時對細(xì)胞核和核仁進(jìn)行活細(xì)胞染色。這些染料可以檢驗由細(xì)胞周期和核糖體發(fā)生等生物過程引起的細(xì)胞內(nèi)分布、運輸和定位的核仁動態(tài)變化。該試劑盒與大多數(shù)熒光檢測系統(tǒng)兼容，包括常規(guī)和共聚焦熒光顯微鏡，以及高內(nèi)涵篩選（HCS）平臺。TOTAL-NUCLEAR-ID? green/red nucleolar/nuclear detection kit專門設(shè)計用于觀察活細(xì)胞中的核仁和細(xì)胞核，潛在應(yīng)用包括監(jiān)測受損核糖體的生物生成、抑制轉(zhuǎn)錄、細(xì)胞周期動力學(xué)和細(xì)胞應(yīng)激，以及核仁蛋白的分布、運輸和動力學(xué)，病毒蛋白的分布，并可能有助于識別癌癥細(xì)胞。
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產(chǎn)品特點
● 同時對活細(xì)胞中的核仁和細(xì)胞核染色
● 高耐光漂白性和抗淬滅，即使在長時間觀察后也能確保強烈一致的熒光信號
● 已驗證在活細(xì)胞成像應(yīng)用中的實用性，對核仁擾動劑的治療有適當(dāng)?shù)姆磻?yīng)性
● 生產(chǎn)嚴(yán)格，以控制和消除非特異性結(jié)果
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實例分析
● NUCLEOLAR-ID?綠色檢測試劑（A）和NUCLEOR-ID?紅色檢測試劑（B）的吸收和發(fā)射光譜
● 用NUCLEOLAR-ID?綠色檢測試劑染色的細(xì)胞在核仁內(nèi)顯示出最大的熒光信號，在整個細(xì)胞核和細(xì)胞質(zhì)中顯示出微弱的熒光（A）。當(dāng)用低劑量放線菌素D處理細(xì)胞時，觀察到核仁染色丟失（B）。與較弱的細(xì)胞質(zhì)染色相比，使用NUCLEOLAR-ID?紅色檢測試劑進(jìn)行反染色有助于突出核仁。
● 用TOTAL-NUCLEAR-ID? Green/Red Nucleolar/ Nuclear Detection Kit對U2OS活細(xì)胞進(jìn)行染色，通過熒光顯微鏡觀察細(xì)胞核（紅色）和核仁（黃綠色）的染色結(jié)果。作為一種RNA染料，NUCLEOLAR-ID?綠色染料也顯示出一些細(xì)胞質(zhì)染色，但NUCLEOR-ID?紅色染料有助于在高亮顯示的紅色細(xì)胞核熒光信號范圍內(nèi)，將核仁明確識別為綠色熒光信號。
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● U2OS細(xì)胞中染色為紅色的細(xì)胞核和染色為綠色的核仁的3D重建
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產(chǎn)品詳情：
| 產(chǎn)品名稱 | TOTAL-NUCLEAR-ID? green/red nucleolar/nuclear detection kit |
| 產(chǎn)品貨號 | ENZ-51006-500 |
| 產(chǎn)品規(guī)格 | 500 assays |
| 質(zhì)量控制 | A sample from each lot of TOTAL-NUCLEAR-ID? Green/ Red Nucleolus/Nucleus Detection Kit is used to stain HeLa cells using the procedures described in the user manual. Cells stained with NUCLEOLAR-ID? Green Detection Reagent show maximal fluorescence signal within the nucleoli, with only faint fluorescence throughout the nucleus and cytoplasm. NUCLEAR-ID? Red Detection Reagent maximally stains the DNA in the cell nucleus. Cells induced by actinomycin D show reduction of nucleolar signal. |
| 應(yīng)用 | Fluorescence microscopy, Fluorescent detection
For visualizing nucleoli and nuclei simultaneously in living cells. |
| 短期儲存 | -20°C |
| 長期儲存 | -80°C |
| 組分 | NUCLEOLAR-ID? Green Detection Reagent, 50 μL
NUCLEAR-ID? Red Detection Reagent, 50 μL
Actinomycin D Control, 125 μg
10X Assay Buffer, 15 mL |
| 產(chǎn)品說明 | The TOTAL-NUCLEAR-ID? green/red nucleolar/nuclear detection kit is a member of the CELLESTIAL? product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL? reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required. |
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部分產(chǎn)品文獻(xiàn)引用
1. Guanidinylated bioresponsive poly(amido amine)s designed for intranuclear gene delivery: J. Yu, et al.; Int. J. Nanomedicine 11, 4011 (2016), Application(s): Confocal microscopy using MCF7 cells
2. Dose-dependent role of the cohesin complex in normal and malignant hematopoiesis: A.D. Viny, et al.; J. Exp. Med. 212, 1819 (2015), Application(s): Confocal microscopy
3. Small-molecule multi-targeted kinase inhibitor RGB-286638 triggers P53-dependent and -independent anti-multiple myeloma activity through inhibition of transcriptional CDKs: D. Cirstea, et al.; Leukemia 27, 2366 (2013), Application(s): Nucleolar segregation analysis by immunofluorescence
4. Mismatch in mechanical and adhesive properties induces pulsating cancer cell migration in epithelial monolayer: M.H. Lee, et al.; Biophys. J. 102, 2731 (2012), Application(s): Detection of nuclei and nucleoli in breast cell lines using fluorescence microscopy
5. AFM stiffness nanotomography of normal, metaplastic and dysplastic human esophageal cells: A. Fuhrmann, et al.; Phys. Biol. 8, 015007 (2011), Application(s): Fluorescence lifetime imaging of normal and dysplasic esophageal cells
6. Strategies of actin reorganisation in plant cells: A.P. Smertenko, et al.; J. Cell Sci. 123, 3019 (2010), Application(s): Staining of plant cells
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