靈敏的ELISA試劑盒，能夠檢測(cè)任何物種的培養(yǎng)上清液和血漿中的12(S)-HETE
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HETE是由脂氧合酶代謝花生四烯酸產(chǎn)生的副產(chǎn)物。12(S)-HETE是12(S)-氫過氧四烯酸（12(S)-HpETE）還原的立體特異性羥基產(chǎn)物，其本身是花生四烯酸的12-脂氧酶代謝產(chǎn)物。12(S)-HETE對(duì)多形核白細(xì)胞和血管平滑肌細(xì)胞有趨化作用和化學(xué)促活作用，它也作為血管緊張素-II誘導(dǎo)的醛固酮產(chǎn)生的第二信使。12(S)-HETE與氧化應(yīng)激、炎癥、癌癥、動(dòng)脈粥樣硬化和心血管疾病等相關(guān)，有研究表明，12(S)-HETE參與抑制腎素的產(chǎn)生，刺激胰腺組織分泌胰島素，誘導(dǎo)內(nèi)皮細(xì)胞收縮和腫瘤細(xì)胞粘附。
Enzo Life Sciences的12S-羥基二十碳四烯酸比色法ELISA試劑盒，可在5小時(shí)內(nèi)得到檢測(cè)結(jié)果，在405nm處讀取OD值。檢測(cè)快速，準(zhǔn)確性高，可靠性好。欣博盛生物作為Enzo Life Sciences授權(quán)中國一級(jí)代理，可提供該產(chǎn)品，歡迎咨詢。
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產(chǎn)品特點(diǎn)
◆ 高通量，僅需5小時(shí)即可獲得多達(dá)39份樣本（含復(fù)孔）的結(jié)果
◆ 高靈敏度，可測(cè)量低至146 pg/ml的12(S)-HETE
◆ 長期和多批次驗(yàn)證的可靠且可重復(fù)的實(shí)驗(yàn)結(jié)果
◆ 易于使用的顏色編碼試劑和預(yù)包被板，減少了錯(cuò)誤并節(jié)省了實(shí)驗(yàn)時(shí)間
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產(chǎn)品信息
產(chǎn)品貨號(hào) | ADI-900-050/ ADI-901-050 |
產(chǎn)品名稱 | 12(S)-HETE ELISA kit（12S-羥基二十碳四烯酸ELISA試劑盒） |
規(guī)格 | 96?wells/ 5x96 wells |
其他名稱 | 12(S)-hydroperoxy tetraenoic eicosatetraenoic acid |
靈敏度 | 146 pg/ml |
檢測(cè)范圍 | 195 - 50,000 pg/ml |
應(yīng)用 | ELISA, Colorimetric detection |
檢測(cè)波長 | 405 nm |
適用樣本類型 | 細(xì)胞培養(yǎng)上清和血漿樣本（血清，腎間質(zhì)液和組織樣本有文獻(xiàn)報(bào)道） |
適用種屬 | 不限種屬 |
使用/穩(wěn)定性 | Store all components at +4°C, except standard and conjugate at -20°C. |
運(yùn)輸 | Blue Ice |
試劑盒組分 | GxR IgG Microtiter plate, Conjugate, Antibody, Assay buffer, Wash buffer ?concentrate, Standard, pNpp Substrate, Stop solution |
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標(biāo)準(zhǔn)曲線示例
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部分產(chǎn)品引用文獻(xiàn)
1.?Leukotriene B4 receptor-2 contributes to KRAS-driven lung tumor formation by promoting interleukin-6-mediated inflammation: J.H. Jang, et al.; Exp. Mol. Med. 53, 1559 (2022)
2.?Development of heart failure with preserved ejection fraction in type 2 diabetic mice is ameliorated by preserving vascular function: M. Otto, et al.; Life Sci. 284, 119925 (2021)
3.?12(S)-HETE Mediates Diabetes Induced Endothelial Dysfunction by Activating Intracellular Endothelial Cell TRPV1: M. Otto, et al.; J. Clin. Invest. 130, 4999 (2020)
4.?Arachidonate 12-Lipoxygenase and 12-Hydroxyeicosatetraenoic acid contribute to stromal aging-induced progression of pancreatic cancer: E.H. Sarsour, et al.; J. Biol. Chem. 295, 6946 (2020)
5.?A Mediterranean Diet Rich in Extra-Virgin Olive Oil Is Associated with a Reduced Prevalence of Nonalcoholic Fatty Liver Disease in Older Individuals at High Cardiovascular Risk: X. Pintó, et al.; J. Nutr. 149, 1920 (2019)
6.?Ablation of TRPV1 Elevates Nocturnal Blood Pressure in Western Diet-fed Mice: B. Zhong, et al.; Curr. Hypertens. Rev. 15, 144 (2019)
7.?Mediatory roles of leukotriene B4 receptors in LPS-induced endotoxic shock: S.Y. Kwon, et al.; Sci. Rep. 9, 5936 (2019)
8.?5‐/12‐Lipoxygenase‐linked cascade contributes to the IL‐33‐induced synthesis of IL‐13 in mast cells, thus promoting asthma development: M. Ro, et al.; Allergy 73, 350 (2018)
9.?Intravasation of SW620 colon cancer cell spheroids through the blood endothelial barrier is inhibited by clinical drugs and flavonoids in vitro: S. Holzner, et al.; Food Chem. Toxicol. 111, 114 (2018)
10.?Mice lacking L-12/15-lipoxygenase show increased mortality during kindling despite demonstrating resistance to epileptogenesis: M.A. Kanzler, et al.; Epilepsia Open 3, 255 (2018)
11.?Changes in platelet function with inflammation in patients undergoing vascular surgery: B. Olechowski, et al.; Platelets 30, 190 (2017)
12.?12(S)-HETE increases intracellular Ca2+ in lymph-endothelial cells disrupting their barrier function in vitro; stabilization by clinical drugs impairing calcium supply: C.H Nguyen, et al.; Cancer Lett. 380, 174 (2016)
13.?12-Lipoxygenase Inhibition on Microalbuminuria in Type-1 and Type-2 Diabetes Is Associated with Changes of Glomerular Angiotensin II Type 1 Receptor Related to Insulin Resistance: H.Z. Xu, et al.; Int. J. Mol. Sci. 17, E684 (2016)
14.?Cancer cell-derived 12(S)-HETE signals via 12-HETE receptor, RHO, ROCK and MLC2 to induce lymph endothelial barrier breaching: C.H. Nguyen, et al.; Br. J. Cancer 115, 364 (2016)
15.?12/15-Lipoxygenase metabolites of arachidonic acid activate PPARγ: a possible neuroprotective effect in ischemic brain: L. Sun, et al.; J. Lipid. Res. 56, 502 (2015)
16.?Lobatin b inhibits NPM/ALK and NF-κB attenuating anaplastic-large-cell-lymphomagenesis and lymphendothelial tumour intravasation: I. Kiss, et al.; Cancer Lett. 356, 994 (2015)
17.?MyD88-BLT2-dependent cascade contributes to LPS-induced interleukin-6 production in mouse macrophage: A.J. Lee, et al.; Exp. Mol. Med. 47, e156 (2015)
18.?Pharmacologic blockade of 12/15-lipoxygenase ameliorates memory deficits, Aβ and tau neuropathology in the triple-transgenic mice: J. Chu, et al.; Mol. Psychiatry 20, 1329 (2015)
19.?Selective inhibition of 12-lipoxygenase protects islets and beta cells from inflammatory cytokine-mediated beta cell dysfunction: D.A. Taylor-Fishwick, et al.; Diabetologia 58, 549 (2015)
20.?Transient postnatal fluoxetine decreases brain concentrations of 20-HETE and 15-epi-LXA4, arachidonic acid metabolites in adult mice: Z.X. Yuan, et al.; Prostaglandins Leukot. Essent. Fatty Acids 101, 9 (2015)
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