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15-deoxy-Δ12,14-PGJ2 ELISA kit——ENZO熱銷(xiāo)產(chǎn)品

2023-09-01
瀏覽次數(shù): 34

首款上市的15-d-PGJ2 ELISA試劑盒,可用于類(lèi)花生酸研究

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15-deoxy-Δ12,14-PGJ2(15-d-PGJ2)是PGD2的最終脫水產(chǎn)物之一,通過(guò)中間體Δ12-PGJ2形成。生理?xiàng)l件下,15-d-PGJ2存在于體液中,濃度介于10^(-12)至10^(-9)M,但在感染和炎癥等應(yīng)激條件下會(huì)急劇增加。在細(xì)胞類(lèi)型和濃度的影響下,15-d-PGJ發(fā)揮促進(jìn)和抗炎癥作用,其中許多作用是通過(guò)與過(guò)氧化物酶體增殖劑激活受體(PPAR-γ)的γ異構(gòu)體結(jié)合而介導(dǎo)的。15-d-PGJ2似乎通過(guò)一種與PPAR-γ無(wú)關(guān)的機(jī)制誘導(dǎo)HO-1的表達(dá),該機(jī)制由活性氧(ROS)的產(chǎn)生介導(dǎo)。

Enzo Life Sciences的15-脫氧-Δ12,14-前列腺素J2 ELISA試劑盒是一種比色競(jìng)爭(zhēng)法酶聯(lián)免疫檢測(cè)試劑盒,可在5小時(shí)內(nèi)得到檢測(cè)結(jié)果,相比GC/MS 分析節(jié)省時(shí)間且耗時(shí)少得多。欣博盛生物作為Enzo Life Sciences授權(quán)中國(guó)一級(jí)代理,可提供該產(chǎn)品,歡迎咨詢(xún)。

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產(chǎn)品特色

◆ 靈敏度高,可測(cè)量低至36.8 pg/ml的15-deoxy-Δ12,14-PGJ2

◆?檢測(cè)快速,較GC/MS分析耗時(shí)少得多

◆?易于使用的即用型液體顏色編碼試劑,可減少錯(cuò)誤

◆?優(yōu)于半定量Western blot分析的完全定量檢測(cè)

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產(chǎn)品信息

產(chǎn)品貨號(hào)

ADI-900-023/ ADI-901-023

產(chǎn)品名稱(chēng)

15-deoxy-Δ12,14-PGJ2 ELISA kit(15-脫氧-Δ12,14-前列腺素J2 ELISA試劑盒)

規(guī)格

96 wells/ 5x96 wells

其他名稱(chēng)

15-deoxy-Δ12,14-Prostaglandin J2

靈敏度

36.8 pg/ml

檢測(cè)范圍

195 - 200,000 pg/ml

檢測(cè)時(shí)間

5 hours

應(yīng)用

ELISA, Colorimetric detection

檢測(cè)波長(zhǎng)

405 nm

樣本類(lèi)型

細(xì)胞培養(yǎng)上清,血漿,唾液和尿液

適用種屬

不限種屬

試劑盒組分

GxR IgG Microtiter plate, Conjugate, Antibody, Assay buffer, Wash buffer concentrate,?

Standard, pNpp Substrate, Stop solution

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標(biāo)曲示例

15-deoxy-Δ12,14-PGJ2 ELISA kit——ENZO熱銷(xiāo)產(chǎn)品?

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部分產(chǎn)品引用文獻(xiàn)

1.?Redox aspects of cytotoxicity and anti-neuroinflammatory profile of chloroquine and hydroxychloroquine in serum-starved BV-2 microglia: L. Ra?ková, et al.; Toxicol. Appl. Pharmacol. 447, 116084 (2022)

2.?Fluoxetine‐induced hepatic lipid accumulation is mediated by prostaglandin endoperoxide synthase 1 and is linked to elevated 15‐deoxy‐Δ12, 14PGJ2: A. Ayyash, et al.; J. Appl. Toxicol. 4272, 1002 (2021)

3.?Oxylipin biosynthesis reinforces cellular senescence and allows detection of senolysis: C.D. Wiley, et al.; Cell Metab. 33, 1124 (2021)

4.?Cocaine-mediated circadian reprogramming in the striatum through dopamine D2R and PPARγ activation: K. Brami-Cherrier, et al.; Nat. Commun. 11, 4448 (2020)

5.?Thymopentin improves the survival of septic mice by promoting the production of 15-deoxy-prostaglandin J2 and activating the PPARγ signaling pathway: Y. Zhang, et al.; FASEB J. 34, 11772 (2020)

6.?Apolipoprotein D overexpression alters hepatic prostaglandin and omega fatty acid metabolism during the development of a non-inflammatory hepatic steatosis: F. Desmarais, et al.; Biochim. Biophys. Acta Mol. Cell Biol. Lipids 1864, 522 (2019)

7.?Association of chronic inflammation and perceived stress with abnormal functional connectivity in brain areas involved with interoception in hepatitis C patients: G. Oriolo, et al.; Brain Behav. Immun. 80, 204 (2019)

8.?Programming of macrophages by UV-irradiated apoptotic cancer cells inhibits cancer progression and lung metastasis: Y.B. Kim, et al.; Cell. Mol. Immunol. 16, 851 (2019)

9.?Arachidonic acid induces ARE/Nrf2-dependent heme oxygenase-1 transcription in rat brain astrocytes: C.C. Lin, et al.; Mol. Neurobiol. 55, 3328 (2018)

10.?Bone-marrow-derived mesenchymal stem cells inhibit gastric aspiration lung injury and inflammation in rats: J. Zhou, et al.; J. Cell Mol. Med. 20, 1706 (2016)

11.?Implications of a genetically determined nitric oxide deficit for endothelial cell-leukocyte interaction and cardiovascular disease: I. Kadiyska; (2016)

12.?CRTH2 is a critical regulator of neutrophil migration and resistance to polymicrobial sepsis: M. Ishii, et al.; J. Immunol. 188, 5655 (2012)

13.?Choroidal involution is a key component of oxygen-induced retinopathy: Z. Shao, et al.; Invest. Ophthalmol. Vis. Sci. 52, 6238 (2011)

14.?Response of chondrocytes to shear stress: antagonistic effects of the binding partners Toll-like receptor 4 and caveolin-1: P. Wang, et al.; FASEB J. 25, 3401 (2011)

15.?The myocardial infarct size-limiting effect of sitagliptin is PKA-dependent, whereas the protective effect of pioglitazone is partially dependent on PKA: Y. Ye, et al.; Am. J. Physiol. Heart Circ. Physiol. 298, H1454 (2010)

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15-deoxy-Δ12,14-PGJ2 ELISA kit——ENZO熱銷(xiāo)產(chǎn)品

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