Enzo Life Sciences的ROS-ID?Hypoxia/Oxidative stress detection kit專門用于使用熒光顯微鏡或流式細(xì)胞術(shù)對(duì)活細(xì)胞（懸浮和貼壁）中的缺氧和氧化應(yīng)激水平進(jìn)行功能性檢測(cè)。該試劑盒包含能夠檢測(cè)缺氧狀態(tài)（紅色）和氧化應(yīng)激水平（綠色）的熒光探針。

檢測(cè)缺氧的染料（紅色）利用缺氧細(xì)胞中存在的硝基還原酶活性，將硝基基團(tuán)轉(zhuǎn)化為羥胺（NHOH）和氨基（NH2）并釋放出熒光探針。

氧化應(yīng)激檢測(cè)試劑（綠色）是一種非熒光的、可透過(guò)細(xì)胞的總ROS檢測(cè)染料，可與多種活性物質(zhì)直接反應(yīng)?？墒褂门鋫錁?biāo)準(zhǔn)熒光素（490/525 nm）和德克薩斯紅（596/670 nm）濾光片的寬場(chǎng)熒光顯微鏡、共聚焦顯微鏡或配備藍(lán)色（488 nm）激光的流式細(xì)胞儀進(jìn)行細(xì)胞分析。

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產(chǎn)品特點(diǎn)

● 高靈敏度和特異性的熒光探針，可用于檢測(cè)活細(xì)胞的缺氧和氧化應(yīng)激

●?可用于分析貼壁或懸浮細(xì)胞系

●?試劑盒內(nèi)包含整套試劑，包括ROS和缺氧誘導(dǎo)劑

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實(shí)驗(yàn)示例

圖1.?檢測(cè)人HeLa和HL-60細(xì)胞中的缺氧和氧化應(yīng)激水平。用缺氧誘導(dǎo)劑（DFO）和ROS誘導(dǎo)劑（pyocyanin）處理細(xì)胞。每個(gè)象限的數(shù)字反映了細(xì)胞（群體）的百分比。結(jié)果表明，缺氧和氧化應(yīng)激染料具有特異性。

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圖2.使用不同試劑誘導(dǎo)HeLa細(xì)胞發(fā)生缺氧和氧化應(yīng)激反應(yīng)。缺氧探針在缺氧條件下被細(xì)胞硝基還原酶轉(zhuǎn)化后觀察到紅色熒光。

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圖3.氧化應(yīng)激（A）和缺氧（B）檢測(cè)染料的吸收峰和發(fā)射峰分別為504nm/524nm和580nm/595nm。這些染料可以用488nm的氬離子激光器激發(fā)，并在流式細(xì)胞儀上的FL1通道（氧化應(yīng)激染料）和FL3通道（缺氧紅染料）中檢測(cè)。

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產(chǎn)品信息

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部分產(chǎn)品引用文獻(xiàn)

1.?Dimethyloxalylglycine (DMOG), a Hypoxia Mimetic Agent, Does Not Replicate a Rat Pheochromocytoma (PC12) Cell Biological Response to Reduced Oxygen Culture: R. Chen, et al.; Biomolecules 12, 541 (2022)

2.?Hydrogel microcapsules containing engineered bacteria for sustained production and release of protein drugs: C. Han, et al.; Biomaterials 287, 121619 (2022)

3.?Inhibiting autophagy flux and DNA repair of tumor cells to boost radiotherapy of orthotopic glioblastoma: Q. Xu, et al.; Biomaterials 280, 121287 (2022)

4.?Intracellular glucose starvation affects gingival homeostasis and autophagy: R. Li, et al.; Sci. Rep. 12, 1230 (2022)

5.?Intrinsic radical species scavenging activities of tea polyphenols nanoparticles block pyroptosis in endotoxin-induced sepsis: Y. Chen, et al.; ACS Nano 16, 2429 (2022)

6.?Iodinated cyanine dye-based nanosystem for synergistic phototherapy and hypoxia-activated bioreductive therapy: Y. Dong, et al.; Drug Deliv. 29, 238 (2022)

7.?Lipoprotein-biomimetic nanostructure enables tumor-targeted penetration delivery for enhanced photo-gene therapy towards glioma: R. Wang, et al.; Bioact. Mater. 13, 286 (2022)

8.?Microenvironment-driven sequential ferroptosis, photodynamic therapy, and chemotherapy for targeted breast cancer therapy by a cancer-cell-membrane-coated nanoscale metal-organic framework: W.L. Pan ,et al.; Biomaterials 283, 121559 (2022)

9.?Mitochondrial glutathione depletion nanoshuttles for oxygen-irrelevant free radicals generation: A cascaded hierarchical targeting and theranostic strategy against hypoxic tumor: B. Liang, et al.; ACS Appl. Mater. Interfaces 14, 13038 (2022)

10.?Multifunctional Nanosnowflakes for T1-T2 Double-Contrast Enhanced MRI and PAI Guided Oxygen Self-Supplementing Effective Anti-Tumor Therapy: Y. Lv, et al.; Int. J. Nanomedicine 17, 4619 (2022)

11.?Physiologic flow-conditioning limits vascular dysfunction in engineered human capillaries: K. Haase, et al.; Biomaterials 280, 121248 (2022)

12.?Platinum prodrug nanoparticles inhibiting tumor recurrence and metastasis by concurrent chemoradiotherapy: W. Jiang, et al.; J. Nanobiotechnology 20, 129 (2022)

13.?Strategy for improving cell-mediated vascularized soft tissue formation in a hydrogen peroxide-triggered chemically-crosslinked hydrogel: S.Y. Wei, et al.; J. Tissue. Eng. 13, 20417314221084096 (2022)

14.?A cyclic nano-reactor achieving enhanced photodynamic tumor therapy by reversing multiple resistances: P. Liu, et al.; J. Nanobiotechnology 19, 149 (2021)

15.?An albumin-based therapeutic nanosystem for photosensitizer/protein co-delivery to realize synergistic cancer therapy: S.L. Ai, et al.; ACS Appl. Bio. Mater. 4, 4946 (2021)

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