Enzo Life Sciences的ROS-ID??Total ROS/Superoxide detection kit包括兩種熒光染料作為主要成分：非熒光、可滲透細(xì)胞的氧化應(yīng)激檢測(cè)試劑（綠色，Ex/Em 490/525 nm）與多種活性物質(zhì)（過(guò)氧化氫、過(guò)氧亞硝酸根和羥基）直接反應(yīng)，產(chǎn)生綠色熒光產(chǎn)物。超氧化物檢測(cè)試劑（橙色，Ex/Em 550/620 nm）是一種可滲透細(xì)胞的探針，可與超氧化物特異性反應(yīng)，產(chǎn)生橙色熒光產(chǎn)物。試劑盒還提供Pycyanin（ROS誘導(dǎo)劑）和NAC（ROS抑制劑）。
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產(chǎn)品特點(diǎn)
●?用于檢測(cè)活細(xì)胞中超氧化物的產(chǎn)生
●?與細(xì)胞培養(yǎng)基的主要成分（酚紅，F(xiàn)BS和BSA）兼容
●?已通過(guò)顯微鏡、流式細(xì)胞儀和微孔板等應(yīng)用驗(yàn)證
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實(shí)驗(yàn)示例
圖1. 在加入了ROS/超氧化物檢測(cè)試劑并用綠膿菌素處理的 HeLa 細(xì)胞中，通過(guò)熒光顯微鏡對(duì)活性氧的形成進(jìn)行了分析。在綠色通道中監(jiān)測(cè)一般氧化應(yīng)激水平，而在橙色通道中檢測(cè)超氧化物的產(chǎn)生。用 NAC（一種通用的 ROS 抑制劑）預(yù)處理可防止 ROS 的形成。
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圖2. 使用ROS-ID??Total ROS/Superoxide detection kit和ROS清除劑/抑制劑分析用抗霉素A（AMA，特異性超氧化物誘導(dǎo)劑）、叔丁基-過(guò)氧化物（TBHP，特異性過(guò)氧化物誘導(dǎo)劑）和綠膿菌素（通用ROS誘導(dǎo)劑）處理的HeLa細(xì)胞中ROS的產(chǎn)生。使用U-2 OS和CHO K1細(xì)胞獲得了類(lèi)似的結(jié)果。
圖3. 通過(guò)流式細(xì)胞術(shù)分析Hela細(xì)胞中ROS的形成。數(shù)據(jù)以綠膿菌素（ROS/SO誘導(dǎo)劑）、TBHP（ROS 誘導(dǎo)劑）和 AMA（超氧化物誘導(dǎo)劑）處理后的陽(yáng)性百分比表示。綠色柱表示ROS 陽(yáng)性率%，橙色柱表示超氧化物陽(yáng)性率%。
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產(chǎn)品信息
產(chǎn)品貨號(hào) | ENZ-51010 |
產(chǎn)品名稱(chēng) | ROS-ID??Total ROS/Superoxide detection kit |
別名 | Reactive oxygen species / Superoxide |
規(guī)格 | 1*1Kit (200 fluorescence microscopy assays or 50 flow cytometry assays or 2x96 microplate assays) |
使用/穩(wěn)定性 | With proper storage, the kit components are stable up to the date noted on the product label.? Store kit at -20°C in a non-frost free freezer, or -80°C for longer term storage. |
短期保存 | -20°C |
長(zhǎng)期保存 | -80°C |
試劑盒組分 | Oxidative Stress Detection Reagent (Green), 300 nmoles Superoxide Detection Reagent (Orange), 300 nmoles ROS Inducer (Pyocyanin), 1 μmole ROS Inhibitor (N-acetyl-L-cysteine), 2 x 10 mg Wash Buffer Salts, 1 pack |
應(yīng)用 | Flow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS |
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部分產(chǎn)品引用文獻(xiàn)
1.?Conditional Knockout of Hypoxia-Inducible Factor 1-Alpha in Tumor-Infiltrating Neutrophils Protects against Pancreatic Ductal Adenocarcinoma: J.L. Sieow, et al.; Int. J. Mol. Sci. 24, 753 (2023)
2.?Antioxidant effect of nicotinamide mononucleotide in tendinopathy: K. Yamaura, et al.; BMC Musculoskelet. Disord. 23, 249 (2022)
3.?Eicosatetraynoic Acid and Butyrate Regulate Human Intestinal Organoid Mitochondrial and Extracellular Matrix Pathways Implicated in Crohn’s Disease Strictures: I. Jurickova, et al.; Inflamm. Bowel Dis. 28, 988 (2022)
4.?Hirsutella sinensis mycelium attenuates bleomycin-induced pulmonary inflammation and fibrosis in vivo: T.T. Huang, et al.; Sci. Rep. 5, 15282 (2022)
5.?Inhibition of mitoNEET attenuates LPS-induced inflammation and oxidative stress: S. Lee, et al.; Cell Death Dis. 13, 127 (2022)
6.?New glycoconjugation strategies for Ruthenium(II) arene complexes via phosphane ligands and assessment of their antiproliferative activity: D. Iacopini, et al.; Bioorg. Chem. 126, 105901 (2022)
7.?Quercetin treatment protects the Achilles tendons of rats from oxidative stress induced by hyperglycemia: T. Yoshikawa, et al.; BMC Musculoskelet. Disord. 23, 563 (2022)
8.?Areca nut extract (ANE) inhibits the progression of hepatocellular carcinoma cells via activation of ROS production and activation of autophagy: P.L. Wei, et al.; Int. J. Med. Sci. 18, 3452 (2021)
9.?Differential oxidative stress responses and tobacco-specific nitrosamine accumulation in two burley varieties: J. Kurepa, et al.; J. Plant Physiol. 261, 153429 (2021)
10.?Dihydroisotanshinone I induced ferroptosis and apoptosis of lung cancer cells: C.Y. Wu, et al.; Biomed. Pharmacother. 139, 111585 (2021)
11.?Incense smoke-induced oxidative stress disrupts tight junctions and bronchial epithelial barrier integrity and induces airway hyperresponsiveness in mouse lungs: N. Yamamoto, et al.; Sci. Rep. 11, 7222 (2021)
12.?Nano-encapsulation of hydroxytyrosol into formulated nanogels improves therapeutic effects against hepatic steatosis: An in vitro study: E. Mauri, et al.; Mater. Sci. Eng. C Mater. Biol. Appl. 124, 112080 (2021)
13.?Non-viral gene delivery of the oncotoxic protein NS1 for treatment of hepatocellular carcinoma: D. Witzigmann, et al.; J. Control Release 334, 138 (2021)
14.?Polymer-ritonavir derivate nanomedicine with pH-sensitive activation possesses potent anti-tumor activity in vivo via inhibition of proteasome and STAT3 signaling: L. Sivák, et al.; J. Control Release 332, 563 (2021)
15.?Selective striatal cell loss is ameliorated by regulated autophagy of the cortex: K. Cho & G.W. Kim; Life Sci. 282, 119822 (2021)
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