畅享海角平台破解平台-海角社区官网官方入口

您好,歡迎光臨欣博盛生物科技商城!
服務(wù)熱線:400 680 0892
新聞資訊 News

ROS-ID?活性氧/活性氮檢測試劑盒——ENZO熱銷產(chǎn)品

2023-10-23
瀏覽次數(shù): 112

自由基和其他活性種在許多心理和病理生理過程中發(fā)揮著重要作用。自由基一旦在細(xì)胞內(nèi)產(chǎn)生,就會破壞多種細(xì)胞成分,包括蛋白質(zhì)、脂質(zhì)和DNA。然而,在較低濃度下,自由基可以作為細(xì)胞信號傳導(dǎo)中的第二信使。

Enzo Life Sciences的ROS-ID??ROS/RNS detection kit可用熒光顯微鏡直接實時監(jiān)測活細(xì)胞中活性氧與活性氮(ROS/RNS)的產(chǎn)生。該試劑盒中包含三種檢測探針:NO檢測試劑(紅色,Ex/Em 650/670 nm),用于總ROS檢測的氧化應(yīng)激檢測試劑(綠色,Ex/Em 490/525 nm)和超氧化物檢測試劑(橙色,Ex/Em 550/620 nm)。試劑盒內(nèi)還包括陽性對照,綠膿菌素和L-精氨酸,它們分別是ROS和NO產(chǎn)生的常見誘導(dǎo)劑;陰性對照N-乙酰-L-半胱氨酸和c-PTIO則分別是ROS和NO的常見清除劑。通過將三種檢測探針與一組特異性抑制劑、激活劑相結(jié)合,能夠區(qū)分超氧化物、一氧化氮和過氧亞硝酸鹽。

?

產(chǎn)品特點

● 直接監(jiān)測活細(xì)胞中的活性氧和氮化物(ROS/RNS)

●?可區(qū)分超氧化物、一氧化氮和過氧亞硝酸鹽

●?高靈敏度、特異性和準(zhǔn)確性,可用于活細(xì)胞研究

●?與組織培養(yǎng)基的主要成分(酚紅、FBS和BSA)兼容

●?試劑盒內(nèi)包含整套試劑,包括ROS/RNS誘導(dǎo)劑和清除劑

?

實驗示例

ROS-ID?活性氧/活性氮檢測試劑盒——ENZO熱銷產(chǎn)品

圖1. 氧化應(yīng)激檢測試劑(綠色)、超氧化物檢測試劑(藍(lán)色)和NO檢測試劑(紅色)的激發(fā)和發(fā)射光譜。

?

ROS-ID?活性氧/活性氮檢測試劑盒——ENZO熱銷產(chǎn)品

圖2. 使用ROS/RNS檢測試劑盒演示HeLa細(xì)胞中過氧亞硝酸鹽和NO生成的代表性實驗。分析表明,由于NO與超氧化物的反應(yīng),L-精氨酸和綠膿菌素的聯(lián)合處理產(chǎn)生過氧亞硝酸鹽(綠色熒光),但很少產(chǎn)生NO(紅色熒光)。

?

ROS-ID?活性氧/活性氮檢測試劑盒——ENZO熱銷產(chǎn)品

??

產(chǎn)品信息

產(chǎn)品貨號

ENZ-51001-200

產(chǎn)品名稱

ROS-ID??ROS/RNS detection kit

別名

Reactive oxygen species / Reactive nitrogen species

規(guī)格

1*1Kit

長期保存

-80°C

試劑盒組分

NO Detection Reagent (Red), 60μl

Oxidative Stress Detection Reagent (Green), 300 nmoles

Superoxide Detection Reagent (Orange), 300 nmoles

NO Inducer (L-Arginine), 100 μl

ROS Inducer (Pyocyanin), 1 μmole

NO Scavenger (c-PTIO), 400 nmoles

ROS Inhibitor (N-acetyl-L-cysteine), 2x10 mg

10X Wash Buffer, 15 ml

應(yīng)用

Fluorescence microscopy

?

部分產(chǎn)品引用文獻(xiàn)

1.?Hypoxia-induced oxidative stress promotes therapy resistance via upregulation of heme oxygenase-1 in multiple myeloma: K. Abe, et al.; Cancer Med. (2023)

2.?Dihydroartemisinin-transferrin adducts enhance TRAIL-induced apoptosis in triple-negative breast cancer in a P53-independent and ROS-dependent manner: X. Zhou, et al.; Front. Oncol. 11, 789336 (2022)

3.?Intermittent exposure of cultured endothelial cells to physiologically relevant fructose concentrations has a profound impact on nitric oxide production and bioenergetics: M.L. Fiorello, et al.; PLoS One 17, e0267675 (2022)

4.?S1P (Sphingosine-1-Phosphate)-Induced Vasodilation in Human Resistance Arterioles During Health and Disease: B. Katunaric, et al.; Hypertension 79, 2250 (2022)

5.?Extra-mitochondrial citrate synthase initiates calcium oscillation and suppresses age-dependent sperm dysfunction: W. Kang, et al.; Lab. Invest. 100, 583 (2020)

6.?Liposome-encapsulated bacillus calmette-guérin cell wall skeleton enhances antitumor efficiency for bladder cancer in vitro and in vivo via induction of amp-activated protein kinase: Y.M. Whang, et al.; Cancers (Basel) 12, 3679 (2020)

7.?Mesenchymal stem cell conditioned medium ameliorates diabetic serum-induced insulin resistance in 3T3-L1 cells: A. Sanap, et al.; Chronic Dis. Transl. Med. 7, 47 (2020)

8.?Isoorientin derived from Gentiana veitchiorum Hemsl. flowers inhibits melanogenesis by down-regulating MITF-induced tyrosinase expression: Q. Wu, et al.; Phytomedicine 57, 129 (2019)

9.?Low intensity ultrasound reduces high glucose reduced nitric oxide generation in retinal pigment epithelial cells: M.B. Karmacharya, et al.; Ultrasound Med. Biol. 44, 647 (2018)

10.?HEK-293 secretome attenuates kainic acid neurotoxicity through insulin like growth factor-phosphatidylinositol-3-kinases pathway and by temporal regulation of antioxidant defense machineries: C. Venugopal, et al.; Neurotoxicology 69, 189 (2017)

11.?Selective enhancing effect of metal ions on mutagenicity: N. Fujii, et al.; Genes Environ. 38, 21 (2016)

12.?Cell-to-cell diffusion of glucose in the mammalian heart is disrupted by high glucose. Implications for the diabetic heart: W.C. De Mello ; Exp. Cell Res. 334, 239 (2015)

13.?Increased Proinflammatory Cytokine Production and Decreased Cholesterol Efflux Due to Downregulation of ABCG1 in Macrophages Exposed to Indoxyl Sulfate: K. Matsuo, et al. ; Toxins (Basel) 7, 3155 (2015)

14.?Regulation of gene expression by tobacco product preparations in cultured human dermal fibroblasts: G.E. Malpass, et al.; Toxicol. Appl. Pharmacol. 279, 211 (2014)

15.?Enterococcus faecalis infection causes inflammation, intracellular oxphos-independent ROS production, and DNA damage in human gastric cancer cells: J.A. Strickertsson, et al.; PLoS One 8, e63147 (2013)

?

?

?

Epicypher熱銷產(chǎn)品——CUTANA? pAG-Tn5 for CUT&Tag

詳情請咨詢Enzo Life Sciences金牌代理——欣博盛生物

全國服務(wù)熱線: 4006-800-892 ? ? ??郵箱: market@neobioscience.com?

深圳: 0755-26755892 ? ? ? ??北京: 010-88594029 ???????????

廣州:020-87615159????????? ?上海: 021-34613729

代理品牌網(wǎng)站: www.yuebanme.com?

自主品牌網(wǎng)站: www.neobioscience.ne


分享到:
關(guān)注欣博盛公眾號
Copyright ?2021 - 2023 深圳欣博盛生物科技有限公司
  網(wǎng)站地圖
犀牛云提供企業(yè)云服務(wù)