甲基化及去甲基化檢測(cè)試劑盒
DNA甲基化(methylation),即將一個(gè)甲基基團(tuán)轉(zhuǎn)移到DNA上的化學(xué)過程,是基因轉(zhuǎn)錄抑制或基因沉默的主要方式。DNA甲基化修飾特異的發(fā)生于CpG位點(diǎn)。甲基基團(tuán)的插入改變了DNA的外形和結(jié)構(gòu),可能會(huì)直接阻止DNA的識(shí)別和轉(zhuǎn)錄因子的結(jié)合,或者吸引其他轉(zhuǎn)錄因子優(yōu)先與DNA結(jié)合而干擾轉(zhuǎn)錄因子的可接近性。目前已經(jīng)鑒定出三個(gè)可與甲基化DNA結(jié)合的蛋白家族,包括MBD結(jié)構(gòu)域蛋白、Kaiso及Kaiso樣蛋白、SRA結(jié)構(gòu)域蛋白。DNA甲基化標(biāo)記通過募集這些蛋白,可促進(jìn)某些組蛋白狀態(tài)的維持,如去乙?;?。而組蛋白甲基轉(zhuǎn)移酶催化組蛋白甲基化,而去甲基化則由組蛋白去甲基化酶完成。Cayman開發(fā)出了一些列甲基化及去甲基化檢測(cè)工具。
?產(chǎn)品名稱 | ?編號(hào) | ?規(guī)格 |
?Demethylase?(Jumonji-type)?Activity?Assay?Kit | ?700390 | ?96?wells |
?Demethylase?(LSD-type)?Activity?Assay?Kit | ?700400 | ?96?wells |
?DNA?Methylation?EIA?Kit | ?589324 | ?96?wells?or?480?wells |
?GLP?SAM-ScreenerTM?Assay?Kit | ?600570 | ?384?wells?or?1920?wells |
?JMJD2A?Inhibitor?Screening?Assay?Kit | ?700360 | ?96?wells |
?JMJD2D?Inhibitor?Screening?Assay?Kit | ?700370 | ?96?wells |
?LSD1?Inhibitor?Screening?Assay?Kit | ?700120 | ?96?wells |
?Methyltransferase?Colorimetric?Assay?Kit | ?700140 | ?96?wells |
?Methyltransferase?Fluorometric?Assay?Kit | ?700150 | ?96?wells |
?MLL1?SAM-ScreenerTM?Assay?Kit | ?600580 | ?384?wells?or?1920?wells |
?SET7/9?Methyltransferase?Inhibitor?Screening?Assay?Kit | ?700270 | ?96?wells |
?SET7/9?SAM-ScreenerTM?Assay?Kit | ?600490 | ?384?wells?or?1920?wells |
?SET8?Methyltransferase?Inhibitor?Screening?Assay?kit | ?700350 | ?96?wells |
乙?;叭ヒ阴;瘷z測(cè)試劑盒
乙?;侵笇⒁粋€(gè)乙酰基(CH3CO)增加到有機(jī)化合物上。組蛋白乙?;D(zhuǎn)移酶(Histone?acetyltranferases,HATs)催化乙酰基從乙酰輔酶A(CoA)轉(zhuǎn)移到賴氨酸殘基側(cè)鏈的末端胺上。由于許多HATs也乙?;渌鞍椎馁嚢彼?縮寫為’K’),因此多數(shù)HATs也被命名為KATs。雖然大約20種的人HATs都有一個(gè)共同的酶催化能力,一些HATs還作用于相同的組蛋白靶點(diǎn),但實(shí)際上HATs的功能是不同的。重要的是,大多數(shù)HATs除含有乙?;D(zhuǎn)移酶結(jié)構(gòu)域外,還含有不同的結(jié)合結(jié)構(gòu)域。例如,MYST酶具有一個(gè)C2HC型鋅指結(jié)構(gòu)域,可結(jié)合其他分子;而CBP和P300具有溴結(jié)構(gòu)域,可結(jié)合乙酰化賴氨酸殘基。這意味著每個(gè)HATs都具有某種特異性,不論是結(jié)合位點(diǎn)還是乙酰化位點(diǎn)。帶正電荷的賴氨酸轉(zhuǎn)換為不帶電荷的乙?;嚢彼幔q如磷酸化過程中帶負(fù)電荷的膦酸基轉(zhuǎn)移到不帶電荷的氨基酸一樣,改變蛋白的結(jié)構(gòu)以及與其他生物分子的相互作用。例如組蛋白乙?;ǔ4龠M(jìn)效應(yīng)分子的募集,染色質(zhì)構(gòu)象的松弛以及活化轉(zhuǎn)錄等。
?產(chǎn)品名稱 | ?編號(hào) | ?規(guī)格 |
?HAT?Inhibitor?Screening?Assay?Kit | ?10006515 | ?96?wells |
?HDAC?Fluorometric?Activity?Assay?Kit | ?10011563 | ?96?wells |
?HDAC?Cell-Based?Activity?Assay?Kit | ?600150 | ?96?wells |
?HDAC1?Inhibitor?Screening?Assay?Kit | ?10011564 | ?96?wells |
?HDAC8?Inhibitor?Screening?Assay?Kit | ?700230 | ?96?wells |
?SIRT1?Direct?Fluorometric?Screening?Assay?Kit | ?10010401 | ?96?wells |
?SIRT1?FRET-Based?Screening?Assay?Kit | ?10010991 | ?96?wells |
?SIRT2?Direct?Fluorometric?Screening?Assay?Kit | ?700280 | ?96?wells |
?SIRT3?Direct?Fluorometric?Screening?Assay?Kit | ?10011566 | ?96?wells |
?SIRT6?Direct?Fluorometric?Screening?Assay?Kit | ?700290 | ?96?wells |
溴結(jié)構(gòu)域TR-FRET分析試劑盒/Bromodomain?TR-FRET?Assay?Kits
可用于高通量快速鑒定溴結(jié)構(gòu)域/乙?;南嗷プ饔玫囊种苿?。
圖. 溴結(jié)構(gòu)域TR-FRET(時(shí)間分辨率熒光共振能量轉(zhuǎn)移)分析試劑盒原理示意圖。該檢測(cè)利用銪螯合物標(biāo)記的重組溴結(jié)構(gòu)域作為供體,乙酰化的組蛋白肽與鏈親和素-APC偶聯(lián)作為受體。銪螯合物受激發(fā)后釋放光子或傳遞給APC分子。
?產(chǎn)品名稱 | ?編號(hào) | ?規(guī)格 |
?BAZ2B?bromodomain?TR-FRET? ?Assay?Kit | ?600710 | ?384/1920?wells |
?BRD2?bromodomain?1?TR-FRET? ?Assay?Kit? | ?600500 | ?384/1920?wells |
?BRD2?bromodomain?2?TR-FRET? ?Assay?Kit | ?600510 | ?384/1920?wells |
?BRD2?bromodomains?1?and?2 ?TR-FRET?Assay?Kit | ?600810 | ?384/1920?wells |
?BRD3?bromodomain?1?TR-FRET? ?Assay?Kit? | ?600630 | ?384/1920?wells |
?BRD3?bromodomains?1?and?2? ?TR-FRET?Assay?Kit | ?600820 | ?384/1920?wells |
?BRD3?bromodomain?2?TR-FRET? ?Assay?Kit? | ?600640 | ?384/1920?wells |
?BRD4?bromodomain?1?TR-FRET? ?Assay?Kit | ?600520 | ?384/1920?wells |
?BRD4?bromodomain?2?TR-FRET? ?Assay?Kit | ?600530 | ?384/1920?wells |
?BRD4?bromodomains?1?and?2? ?TR-FRET?Assay?Kit | ?600830 | ?384/1920?wells |
?BRDT?bromodomain?1?TR-FRET? ?Assay?Kit | ?600650 | ?384/1920?wells |
?BRG1?bromodomain?TR-FRET? ?Assay?Kit | ?600720 | ?384/1920?wells |
?BRM?bromodomain?TR-FRET? ?Assay?Kit | ?600730 | ?384/1920?wells |
?CBP?bromodomain?TR-FRET? ?Assay?Kit | ?600850 | ?384/1920?wells |
?TAF-1?bromodomain?1?TR-FRET? ?Assay?Kit | ?600870 | ?384/1920?wells |
?TAF-1?bromodomains?1?and?2? ?TR-FRET?Assay?Kit | ?600930 | ?384/1920?wells |