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● 在流式平臺上計數(shù)細(xì)胞
● 利用抗體標(biāo)記細(xì)胞并利用123count eBeads計算該細(xì)胞群數(shù)量
● 可同時并用FVD細(xì)胞存活染料標(biāo)記活細(xì)胞計數(shù)
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123count eBeads?應(yīng)用領(lǐng)域
● 全血細(xì)胞計數(shù)
● 流式細(xì)胞儀計數(shù)功能驗(yàn)證
● 精子濃度計數(shù)
● 可并用細(xì)胞存活染料7-AAD, PI或FVD計數(shù)細(xì)胞存活率
● 使用磁珠細(xì)胞分選試劑盒后計算細(xì)胞分選產(chǎn)率與純度
貨號：01-1234-42 , 100個反應(yīng)
123count eBeads?使用方式
● 加入123count eBeadsTM→選定待計數(shù)的細(xì)胞 →公式計算細(xì)胞濃度。
● 9加入*已知濃度的123count eBeads 進(jìn)未知濃度的細(xì)胞樣本
● 流式細(xì)胞儀同時讀取細(xì)胞與123count eBeads
● 公式計算細(xì)胞濃度如下：
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? Whole blood was stained with?CD45 PE, CD3 FITC and CD4 APC,?and then
??lysed with 1-step?Fix/Lyse Solution (10X)(cat. no. 00-5333). 123count
? eBeads??were added after lysis.?Lymphocytes were gated on?CD45+ cells,
? and then?analyzed?for CD3+ and CD4+ cells (left).?123count eBeads were
? counted?based?on bright fluorescence in?FITC and PerCP-eFluor? 710 (right).
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全血CD4實(shí)際細(xì)胞計數(shù)示范
= (流式細(xì)胞儀顯示細(xì)胞數(shù)量/流式細(xì)胞儀顯示eBead數(shù)量) X eBead標(biāo)示濃度
= (541個細(xì)胞/1009 eBeads) X 998 eBeads/ul
= 535個細(xì)胞/ul
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