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調(diào)節(jié)性T細(xì)胞(Treg)檢測試劑盒

2021-05-17
瀏覽次數(shù): 507
天然CD4+ CD25+調(diào)節(jié)性T細(xì)胞( regulatory Tcell,Treg )為一類具有免疫抑制作用的T細(xì)胞,約占外周CD4+ T細(xì)胞的5~10%。該類細(xì)胞以'主動(dòng)'的方式參與免疫應(yīng)答/免疫耐受的調(diào)控, 不僅參與自身免疫耐受調(diào)節(jié),而且在腫瘤免疫和移植免疫中也具有重要的作用。Foxp3( forkhead box P3,Scurfin)是目前公認(rèn)的Treg細(xì)胞最特征性的標(biāo)志,其表達(dá)及功能與Treg細(xì)胞密切相關(guān)。Foxp3主要表達(dá)于胸腺、脾臟和淋巴結(jié)等淋巴器官和組織,主要通過流式細(xì)胞術(shù)來檢測,美國eBioscience公司是最早擁有Foxp3流式檢測抗體的公司之一,公司通過不斷改進(jìn)和優(yōu)化,建立了一套完美的針對(duì)Foxp3流式檢測特殊的試劑和方案。
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?供貨商
?產(chǎn)品編號(hào)
?產(chǎn)品名稱
?規(guī)格
?成分
?eBioscience
?88-4994-40
?Human/Non-Human Primate Regulatory T?Cell Staining Kit #2?Kit
?Kit?(25 tests)
?FITC anti-human CD4 (OKT4): 25 tests.
?PE anti-human CD25 (BC96): 25 tests.
?APC anti-human Foxp3 (PCH101): 25 tests.
?APC Rat IgG2a isotype control: 25 tests.
?Normal Rat Serum: 100 μl.
?eBioscience Flow Cytometry Staining Buffer: 200 ml.
?eBioscience Fixation/Permeabilization?Concentrate: 30ml.
?eBioscience Fixation/Permeabilization Diluent: 100 ml. ?
?eBioscience Permeabilization Buffer (10X): 100 ml.
?未提供:Isotype?controls?for?anti--
?CD4(mouse?IgG1,?cat.?11‐4714)?and?anti‐
?CD25?(mouse?IgG1,?cat.?12‐4714)
?eBioscience
?88-4999-40
?Human/Non-Human?Primate Regulatory T?Cell?Staining Kit #1Kit
?Kit?(25 tests)
?FITC anti-human CD4 (OKT4): 25 tests.
?APC anti-human CD25 (BC96): 25 tests.
?PE anti-human Foxp3 (PCH101): 25 tests.
?PE Rat IgG2a isotype control: 25 tests.
?Normal Rat Serum: 100 μl.
?eBioscience Flow Cytometry Staining Buffer: 200 ml.
?eBioscience Fixation/Permeabilization Concentrate: 30 ml.
?eBioscience Fixation/Permeabilization Diluent: 100 ml.
?eBioscience Permeabilization Buffer (10X): 100 ml.
?未提供:Isotype?controls?for?anti‐
?CD4?(mouse?IgG1,?cat.?11‐4714)?and?anti‐
?CD25?(mouse?IgG1,?cat.?17‐4714)
?eBioscience
?88-8111-40
?Mouse Regulatory T
?Cell Staining Kit #1 Kit
?Kit?(50 tests)
?FITC anti-mouse CD4 (RM4-5): 50 μg. Use at 0.125 μg/test.
?APC anti-mouse CD25 (PC61.5): 50 μg. Use at 0.06 μg/test.
?PE anti-mouse/rat Foxp3 (FJK-16s): 25 μg. Use at 0.5 μg/test.
?PE Rat IgG2a isotype control: 50 μg. Use at 0.5 μg/test.
?Affinity Purified anti-mouse CD16/32 (Fc Block): 50 μg. Use at 1-5 μg/test.
?eBioscience Flow Cytometry Staining Buffer: 200 ml.
?eBioscience Fixation/Permeabilization Concentrate: 30 ml.
?eBioscience Fixation/Permeabilization Diluent: 100 ml.
?eBioscience Permeabilization Buffer (10X): 100 ml.
?未提供:Isotype controls for anti-
?CD4 (rat IgG2a, cat. 11-4321) and anti-
?CD25 (rat IgG1, cat. 17-4301)
?eBioscience
?88-8115-40
?Mouse Regulatory T
?Cell Staining Kit #3 Kit
?Kit
?FITC anti-mouse CD4 (RM4-5): 50 μg. Use at 0.125 μg/test.
?PE anti-mouse CD25 (PC61.5): 50 μg. Use at 0.06 μg/test.
?PE-Cy5 anti-mouse/rat Foxp3 (FJK-16s): 25 μg. Use at 0.5 μg/test.
?PE-Cy5 Rat IgG2a isotype control: 25 μg. Use at 0.5 μg/test.
?Affinity Purified anti-mouse CD16/32 (Fc Block): 50 μg. Use at 1-5 μg/test
?eBioscience Flow Cytometry Staining Buffer: 200 ml
?eBioscience Fixation/Permeabilization Concentrate: 30 ml.?eBioscience ?
?Fixation/Permeabilization Diluent
: 100 ml.
?eBioscience Permeabilization Buffer (10X): 100 ml.
?未提供:Isotype controls for anti-
?CD4 (rat IgG2a, cat. 11-4321) and anti-
?CD25 (rat IgG1, cat. 12-4301)
?eBioscience
?88-8118-40
?Mouse Regulatory T
?Cell Staining Kit #2 Kit
?Kit
?FITC anti-mouse CD4 (RM4-5): 50 μg. Use at 0.125 μg/test.
?PE anti-mouse CD25 (PC61.5): 50 μg. Use at 0.06 μg/test.
?APC anti-mouse/rat Foxp3 (FJK-16s): 25 μg. Use at 0.5 μg/test.
?APC Rat IgG2a isotype control: 50 μg. Use at 0.5 μg/test.
?Affinity Purified anti-mouse CD16/32 (Fc Block): 50 μg Use at 2-5 μg/test.
?Flow Cytometry Staining Buffer: 200 ml
?Fixation/Permeabilization Concentrate: 30 ml.Fixation/Permeabilization Diluent: 100 ml. Store at 2-8°C.
?Permeabilization Buffer (10X): 100 ml.
?未提供:Isotype controls for anti-
?CD4 (rat IgG2a, Cat. No. 11-4321) and anti-
?CD25 (rat IgG1, Cat. No. 12-4301)
?eBioscience
?88-8995-40
?Human Regulatory T
?Cell Staining Kit #3 Kit
?Kit
?Cocktail of FITC anti-human CD4 (RPA-T4) and PE anti-human CD25 (BC96): 25 tests.
?PE-Cy5 anti-human Foxp3 (PCH101): 25 tests.
?PE-Cy5 Rat IgG2a isotype control: 25 μg.
?Normal Rat Serum: 100 μl.
?eBioscience Flow Cytometry Staining Buffer: 200 ml.
?eBioscience Fixation/Permeabilization ? Concentrate: 30 ml.
?eBioscience Fixation/Permeabilization Diluent: 100 ml.?
?eBioscience Permeabilization Buffer (10X): 100 ml.
?未提供:
?12-4714? Mouse?IgG1?K?Isotype?Control?PE? ? ? ?
?11-4714? Mouse IgG1 K Isotype Control FITC
?eBioscience
?88-8998-40
?Human Regulatory T
?Cell Staining Kit #2Kit
?Kit
?Cocktail of FITC anti-human CD4 (RPA-T4) and PE anti-human CD25 (BC96): 25 tests.
?APC anti-human Foxp3 (PCH101): 25 tests.
?APC Rat IgG2a isotype control: 25 tests.
?Normal Rat Serum: 100 μl.
?eBioscience Flow Cytometry Staining Buffer: 200 ml.
?eBioscience Fixation/Permeabilization Concentrate: 30 ml.?
?eBioscience Fixation/Permeabilization Diluent: 100 ml.
?eBioscience Permeabilization Buffer (10X): 100 ml.
?未提供:
?12-4714? Mouse?IgG1?K?Isotype?Control?PE? ? ?
?11-4714? Mouse IgG1 K Isotype Control FITC
?eBioscience
?88-8999-40
?Human Regulatory T
?Cell Staining Kit Kit
?Kit
?Cocktail of FITC anti-human CD4 (RPA-T4) and APC anti-human CD25 (BC96): 25 tests.
?PE anti-human Foxp3 (PCH101): 25 tests.
?PE Rat IgG2a isotype control: 25 tests.
?Normal Rat Serum: 100 μl.
?eBioscience Flow Cytometry Staining Buffer: 200 ml.
?eBioscience Fixation/Permeabilization Concentrate: 30 ml.
?eBioscience Fixation/Permeabilization Diluent: 100 ml.
?eBioscience Permeabilization Buffer (10X): 100 ml.
?未提供:
?11-4714? Mouse IgG1 K Isotype Control FITC?
?17-4714 ?Mouse?IgG1?K?Isotype?Control?APC
?eBioscience
?88-8996-40
?Human Regulatory T
?Cell Whole Blood
?Staining Kit
?Kit
?eBioscience 1X RBC Lysis Buffer: 200 ml
?eBioscience Flow Cytometry Staining Buffer: 600 ml
?eBioscience Fixation/Permeabilization Concentrate: 30 ml
?eBioscience Fixation/Permeabilization Diluent: 100 ml
?PE anti-human Foxp3 (PCH101): 25 tests
?未提供:
?CD4?抗體和CD25抗體及其同型對(duì)照
?12-4321 Rat IgG2a K Isotype Control PE (eBR2a)


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