EpiCypher是一家為表觀遺傳學(xué)和染色質(zhì)生物學(xué)研究提供高質(zhì)量試劑和工具的專業(yè)制造商。EpiCypher推出的ChIP級(jí)別的Histone H3K27ac Antibody符合EpiCypher的“SNAP-ChIP? Certified”標(biāo)準(zhǔn)，用于ChIP實(shí)驗(yàn)中的特異性和有效靶標(biāo)富集（整板的交叉反應(yīng)性<20%，靶標(biāo)輸入的回收率>5%）。組蛋白H3是核小體中存在的四種蛋白質(zhì)之一，核小體是染色質(zhì)的基本重復(fù)亞基，有147個(gè)堿基對(duì)的DNA纏繞在核心組蛋白（H2A、H2B、H3和H4）的八聚體上。該抗體與H3K27ac結(jié)合，并且在EpiCypher SNAP-ChIP K-AcylStat Panel（EpiCypher 19-3001）中未檢測(cè)到與其他賴氨酸?；娘@著交叉反應(yīng)性。在S28（H3K27acS28ph）磷酸化的情況下，抗體與H3K27ac的結(jié)合在ChIP中受到不同程度的抑制（圖3）。

產(chǎn)品詳情

反應(yīng)種屬: Human, Mouse, Wide Range (Predicted)

宿主來源: Mouse

實(shí)驗(yàn)應(yīng)用: ChIP, ICC/IF

免疫原: A synthetic peptide corresponding to histone H3 acetylated at lysine 27

克隆性: Monoclonal

保存溫度: Stable for 1 year at -20°C from date of receipt

運(yùn)輸溫度: Frozen cold packs.

產(chǎn)品形式: Protein A affinity-purified antibody in PBS pH 7.4, 0.05% sodium azide

驗(yàn)證數(shù)據(jù)

Figure 1:Representative SNAP-ChIP-seq results:?Cumulative histogram plot and heatmap of signal intensity depict H3K27ac ChIP-seq data aligned to annotated transcription start sites (TSS, +/- 3.0 kb; left). Two representative genomic regions depicting H3K27ac peak structure and distribution are shown (right).

Figure 2: SNAP-ChIP-qPCR data. Histone H3K27ac antibody (3 μg) was tested in a native ChIP experiment using chromatin from K562 cells (3 μg) with the SNAP-ChIP K-AcylStat Panel (EpiCypher 19-3001) spiked-in prior to micrococcal nuclease digestion. Specificity (left y-axis) was determined by qPCR for the DNA barcodes corresponding to modified nucleosomes in the SNAP-ChIP panel (x-axis). Black bar represents antibody efficiency (right yaxis; log scale) and indicates percentage of the target immunoprecipitated relative to input. Error bars represent mean ± SEM in replicate ChIP experiments.

訂購(gòu)詳情

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