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Jacalin, Agarose bound, 10 ml/木菠蘿凝集素，瓊脂糖交聯(lián)
品牌： Vectorlabs
產(chǎn)品貨號(hào)： AL-1153-10
規(guī)格： 10 ml

報(bào)價(jià)：
折后價(jià)：

數(shù)量： - +
產(chǎn)品詳情
* 以下信息僅供參考，詳情請(qǐng)以原廠網(wǎng)站為準(zhǔn)
  • 產(chǎn)品名稱(chēng):
    Jacalin, Agarose bound, 10 ml/木菠蘿凝集素，瓊脂糖交聯(lián)
  • 別名:
    木菠蘿凝集素，瓊脂糖交聯(lián)
  • 產(chǎn)品描述:
    Jacalin is a lectin composed of four subunits of approximately 16 kDa each. This lectin appears to bind only O-glycosidically linked oligosaccharides, preferring the structure galactosyl (β-1,3) N-acetylgalactosamine. This structure (the T-antigen) is the oligosaccharide to which peanut agglutinin (PNA) binds. However, unlike PNA, Jacalin will bind a mono- or disialylated form of this structure. This lectin has been used to purify human IgA. The specificity of this lectin also affords the opportunity to localize or isolate glycoproteins with O-glycosidically linked oligosaccharide side chains.Agarose bound* Jacalin is prepared using our affinity-purified lectins. Heat stable, cross-linked 4% agarose beads with a molecular weight exclusion limit of about 2x107 daltons are used as the solid-phase matrix to which the lectins are covalently coupled. The attachment of the lectins to the beads is carefully controlled to preserve lectin activity and minimize conformational changes of the bound lectins that might result in nonspecific ionic or hydrophobic interactions. The technique we have developed to couple lectins to agarose beads inserts a hydrophilic spacer arm between the lectin and the matrix.This coupling method provides several advantages over the traditional cyanogen bromide procedure:Maximum carbohydrate binding activity of the coupled lectins is retainedLinkage is stable over a range of pH valuesConjugated proteins are not leached off the beads by Tris or other routinely used buffersNo residual charges are present after conjugation. This minimizes non-specific binding to the matrix.Our agarose bound lectins are supplied at a constant concentration of lectin per ml of settled beads. The concentration for each lectin is selected to achieve the highest glycoconjugate binding capacity per mg of lectin present in the beads. Each lot is tested for its binding capacity using glycoproteins known to bind the lectin. This provides a guideline for the user and assures the quality of our agarose bound lectins.Inhibiting/Eluting Sugar: 800 mM galactose or 100 mM melibiose*4 mg lectin/ml gel
  • 反應(yīng)種屬:
  • 宿主來(lái)源:
  • 實(shí)驗(yàn)應(yīng)用:
    Glycobiology,Affinity Chromatography
  • 靶標(biāo)/特異性:
  • 同種型:
  • 推薦稀釋度:
    Wash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin. Note:To optimize elution of glycoproteins, wash the gel with 10 column volumes of 175 mM TRIS, pH 7.5, before use.Glycoproteins should be applied in this buffer. Recommended product for eluting glycoconjugates bound to this agarose-lectin: Glycoprotein Eluting Solution, Cat. No. ES-2100. Alternatively, 0.1 M melibiose or 0.8 M galactose in 175 mM TRIS, pH 7.5 can be used.Use of other buffers may result in a reduction in yield of eluted glycoproteins. After use, wash the gel with several column volumes of buffered saline, then resuspend gel in buffered saline containing 0.08% sodium azide for storage.
  • 免疫原:
  • 免疫原種屬:
  • 克隆性:
  • 克隆號(hào):
  • 純化方式:
  • 偶聯(lián):
    Agarose
  • 產(chǎn)品濃度:
  • 表達(dá)系統(tǒng):
  • 陽(yáng)性對(duì)照:
  • 保存溫度:
    2° - 8°C – Do Not Freeze
  • 運(yùn)輸溫度:
    Ambient
  • 細(xì)胞定位:
  • 預(yù)期分子量:
  • 產(chǎn)品形式:
  • 存儲(chǔ)溶液:
    10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.1 mM CaCl2, 20 mM galactose, 20 mM lactose, 0.08% sodium azide
  • 有效期:
  • 產(chǎn)地:
    美國(guó)
  • 外觀:
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  • 檢測(cè)方法:
  • 樣本類(lèi)型:
    10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.1 mM CaCl2, 20 mM galactose, 20 mM lactose, 0.08% sodium azide
  • 產(chǎn)品特點(diǎn):
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