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Expedeon 2019 年新產(chǎn)品 Lightning Link 系列金屬標(biāo)記試劑盒現(xiàn)在有多達(dá)30種啦!
操作步驟
可選標(biāo)記
Lightning-Link??金屬標(biāo)記試劑盒 |
Lightning-Link???139La | Lightning-Link??159Tb |
Lightning-Link???141Pr | Lightning-Link??160Gd |
Lightning ?Link?-142Nd | Lightning-Link??162Dy |
Lightning-Link???143Nd | Lightning-Link??164Dy |
Lightning-Link???144Nd | Lightning-Link??165Ho |
Lightning-Link???146Nd | Lightning Link?-166Er |
Lightning-Link???147Sm | Lightning Link?-167Er |
Lightning-Link???148Nd | Lightning-Link??168Er |
Lightning-Link???149Sm | Lightning-Link??169Tm |
Lightning-Link???151Eu | Lightning-Link??170Er |
Lightning-Link???152Sm | Lightning-Link??171Yb |
Lightning-Link???153Eu | Lightning-Link??172Yb |
Lightning-Link???154Sm | Lightning-Link??174Yb |
Lightning ?Link?-156Gd | Lightning-Link??175Lu |
Lightning-Link???158Gd | Lightning-Link??176Yb |
實(shí)驗(yàn)數(shù)據(jù)
Figure 1. Goat Anti-Rabbit was labeled with Lightning-Link??159Tb at 100 μg single reaction vial and tested with Rabbit IgG in a sandwich ELISA. Detection: Dissociation-enhanced lanthanide fluorescence.
Figure 2. Goat Anti-Rabbit was labeled with a selection of Lightning-Link??metal labels at 10 μg single reaction vials. 2 μg conjugates per well were run on a 4-12% Bis-Tris gel (Expedeon, NBT41212), under non-reducing conditions for 20 minutes at 50V and 1 hour at 120V in 1x MOPS (Expedeon, NXB75500). The gel was stained with InstantBlue? (Expedeon, ISB1L) for 1 hour on the gel rocker. The gel showed no free antibody post conjugation for all conjugates except when the reaction was quenched at t=0, where no conjugation was observed.
Figure 3. X-Ray Fluorescence Lα series peaks used to quantify the metal content in Lightning-Link??Metal Labeling kit.
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