品牌：Mirus

貨號：MIR 2300

品名：TransIT?-LT1 Transfection Reagent

產(chǎn)品信息：

產(chǎn)品類型：轉(zhuǎn)染試劑

規(guī)格：1ml

應(yīng)用：穩(wěn)定轉(zhuǎn)染；病毒生產(chǎn)；干細(xì)胞轉(zhuǎn)染。

儲(chǔ)存條件：4℃

保質(zhì)期：購買產(chǎn)品起1年。

產(chǎn)品介紹：

TransIT?-LT1 Transfection Reagent可用于廣譜DNA遞送-對多種細(xì)胞使用一種轉(zhuǎn)染試劑和協(xié)議；低細(xì)胞毒性-維持細(xì)胞密度，減少實(shí)驗(yàn)偏差；高效傳遞-在大量細(xì)胞中實(shí)現(xiàn)表達(dá)，以獲得實(shí)驗(yàn)成功；提供單?；蚨嗔Ｙ|(zhì)粒-適用于病毒生產(chǎn)等應(yīng)用。

Exceptional Transfection Efficiency in Human Induced Pluripotent Stem Cells (iPSCs) via Reverse Transfection with TransIT?-LT1. The TransIT?-LT1 Transfection Reagent was used to reverse transfect 1.3 x 106 iPS cells with a ZsGreen expressing plasmid (Clontech). Reverse transfections were performed in 6-well plates using 12 μl of TransIT?-LT1 Transfection Reagent to deliver 4 μg of DNA (3:1, reagent: DNA). Cells were visualized 48 hours post-transfection and imaged under a 10X objective with an Olympus IX71? Inverted Microscope. Images are (A) phase contrast and (B) green fluorescence. Cells were assayed 48 hours post-transfection on an Accuri? Cytometer. The histogram (C) shows untransfected cells (black line) compared to cells transfected with plasmid using TransIT?-LT1 (green line).?

High Efficiency Transfection of iCell? Cardiomyocytes Using TransIT?-LT1 Transfection Reagent. iCell? Cardiomyocytes were plated at 20,000 cells/well in a 96 well tissue culture plate coated with 0.1% gelatin. After allowing the cells to recover from thaw, cells were transfected with 100 ng/well of pMAXGFP (Lonza) using TransIT?-LT1 Transfection Reagent with a 2:1 reagent-to-DNA ratio according to the manufacturer’s instructions. Fluorescent images were taken 3 days post transfection using a Olympus IX71? inverted microscope.?

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