褪黑激素(Melatonin，MT)又稱為N-乙?；?/span>-5-甲氧基色胺，在松果體中產(chǎn)生，是調(diào)節(jié)晝夜節(jié)律的主要成分。褪黑激素是由色氨酸通過一系列羥基化和甲基化步驟產(chǎn)生的。褪黑素在肝臟中代謝成6-羥基硫酸褪黑素形式后隨尿液排出體外。褪黑素合成后，儲存在松果體內(nèi)，交感神經(jīng)興奮支配松果體細胞釋放褪黑素。褪黑素的分泌具有明顯的晝夜節(jié)律，白天分泌受抑制，晚上分泌活躍。褪黑素可抑制下丘腦-垂體-性腺軸，使促性腺激素釋放激素、促性腺激素、黃體生成素以及卵泡雌激素的含量均減低，并可直接作用于性腺，降低雄激素、雌激素及孕激素的含量。褪黑激素及其代謝產(chǎn)物具有抗氧化和清除自由基的潛力。還有證據(jù)表明，它們在活性氧蛋白途徑的調(diào)節(jié)中發(fā)揮作用。研究表明，褪黑激素水平與某些癌癥的發(fā)病率可能存在相關(guān)性。唾液可以用來檢測褪黑激素水平。與血清或血漿相比，唾液相對容易獲得，并且不含有血清和血漿中存在的諸多干擾物質(zhì)。唾液中的褪黑激素代表了全身循環(huán)的水平，盡管確切的比例尚未完全量化，公布的數(shù)值范圍為血清值的20-50%。褪黑素在癌癥、睡眠周期、衰老和其他領(lǐng)域的作用也越來越引起人們的關(guān)注，尤其關(guān)注褪黑素受體和循環(huán)褪黑素的生物利用度，以及褪黑素作為膳食補充劑的使用。
ENZO Melatonin ELISA kit是一種比色競爭法免疫檢測試劑盒，可在2小時內(nèi)得到檢測結(jié)果。
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●?高靈敏度，可檢測到低至0.162 ng/ml的褪黑素
●?檢測范圍廣，適用于多種樣本
●?高通量，2小時內(nèi)可獲得多達40個樣品的結(jié)果（帶復(fù)孔）
●?對包括水果勻漿在內(nèi)的各種樣品進行了驗證
產(chǎn)品信息
產(chǎn)品貨號 | ENZ-KIT150-0001 |
產(chǎn)品名稱 | Melatonin ELISA Kit（褪黑素ELISA試劑盒） |
規(guī)格 | 96 wells |
其他名稱 | N-acetyl-5-methoxytryptamine |
靈敏度 | 0.162 pg/ml |
檢測范圍 | 0.08-50 pg/ml |
檢測時間 | 2 hours |
樣本種屬 | Species independent |
樣本類型 | Validated specifically for saliva, serum, plasma, and fruit samples |
保存溫度 | All components of this kit, except the standard and antibody, can be stored at +4°C. The standard and antibody must be stored at -20°C |
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試劑盒組分 | Goat anti-mouse IgG Microtiter Plate, Assay Buffer 10, Standard, Wash Buffer Concentrate, Antibody,?Tracer, Conjugate, TMB Substrate, and Stop Solution |
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| Parallelism of the curves demonstrates that the standard binding characteristics are similar enough to allow the accurate determination of native melatonin levels in diluted samples matrices of animal and plant origin. ? |
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| Typical Melatonin ELISA Data |
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| This experiment shows the normal rise and fall of melatonin levels during the course of the day, and this kit’s ability to measure the biological relevant amounts. This experiment shows the normal rise and fall of melatonin levels during the course of the day, and this kit’s ability to measure the biological relevant amounts. |
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部分產(chǎn)品文獻引用
1. Exogenous SA Applications Alleviate Salinity Stress via Physiological and Biochemical changes in St John’s Wort Plants: E.H. Kwon, et al.; Plants 12, 310 (2023)
2. The circadian system in cystic fibrosis mice is regulated by histone deacetylase 6: E. Barbato, et al.; Am. J. Physiol. Cell Physiol. 323, C1112 (2022)
3. Tubulin Polymerization Promoting Protein Affects the Circadian Timing System in C57Bl/6 Mice: E. Barbato, et al.; J. Circadian Rhythms 19, 5 (2021)
4. Administration of melatonin improves testicular blood flow, circulating hormones, and semen quality in Shiba goats: H. Samir, et al.; Theriogenology 146, 111 (2020), Application(s): Goat plasma
5. Melatonin activates cell death programs for the suppression of uterine leiomyoma cell proliferation: P.H. Lin, et al.; J. Pineal Res. 68, e12620 (2020), Application(s): Mouse serum
6. Melatonin decreases cocaine-induced locomotor activity in pinealectomized rats: S. Barbosa-Méndez & A. Salzar-Juárez; Braz. J. Psychiatry 42, 295 (2020), Application(s): Rat plasma
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