Enzo Life Sciences公司的NUCLEAR-ID??Blue DNA stain (GFP-CERTIFIED?)和NUCLEAR-ID ??Red DNA Stain是細(xì)胞滲透性染料，適用于活細(xì)胞的細(xì)胞核染色。這系列染料為通過(guò)流式細(xì)胞術(shù)研究細(xì)胞周期進(jìn)程的誘導(dǎo)和抑制提供了一種簡(jiǎn)便方法。該試劑在活細(xì)胞研究中的潛在應(yīng)用包括確定細(xì)胞DNA含量和細(xì)胞周期分布、檢測(cè)生長(zhǎng)模式的變化、監(jiān)測(cè)細(xì)胞凋亡以及評(píng)估腫瘤細(xì)胞行為和抑制基因機(jī)制。
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NUCLEAR-ID??Red DNA stain
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產(chǎn)品特點(diǎn)
● 遠(yuǎn)紅色熒光特異性DNA染料
●?穩(wěn)定且純度高
●?可分析活細(xì)胞、可滲透細(xì)胞和固定細(xì)胞
●?無(wú)光漂白作用
●?無(wú)需RNase處理
●?與GFP和FITC兼容
●?不需要紫外線激光源激發(fā)
●?經(jīng)驗(yàn)證適用于各種細(xì)胞密度
●?快速且易于使用
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實(shí)例分析
在37°C下用終濃度為4000x或2000x的NUCLEAR-ID ??Red DNA Stain或同等μM濃度的競(jìng)爭(zhēng)品牌染料對(duì)細(xì)胞進(jìn)行染色，并在染色后輕輕洗滌。在15分鐘和24小時(shí)對(duì)細(xì)胞成像，結(jié)果顯示NUCLEAR-ID ??Red DNA Stain在更低的濃度就能顯現(xiàn)dsDNA。
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產(chǎn)品詳情：
產(chǎn)品名稱 | NUCLEAR-ID??Red DNA stain |
產(chǎn)品貨號(hào) | ENZ-52406 |
產(chǎn)品規(guī)格 | 200μl |
最大激發(fā)波長(zhǎng) | 566nm |
最大發(fā)射波長(zhǎng) | 650nm |
純度 | ≥93%（HPLC） |
質(zhì)量控制 | 1.Absorption peak of NUCLEAR-ID??Red dye: λmax = 566 ± 4 nm 2.% purity of NUCLEAR-ID??Red dye by HPLC: ≥93% |
應(yīng)用 | Flow Cytometry, Fluorescent detection |
短期儲(chǔ)存 | -20°C |
長(zhǎng)期儲(chǔ)存 | -80°C |
產(chǎn)品說(shuō)明 | The NUCLEAR-ID??Red DNA Stain is a member of the CELLESTIAL??product line, reagents and assay? kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis? applications. CELLESTIAL??reagents and kits are optimal for use in demanding imaging applications, such? as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required. |
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部分產(chǎn)品文獻(xiàn)引用
1.?CD200R1L is a functional evolutionary conserved activating receptor in human neutrophils: M.I.P. Ramos, et al.; J. Leukoc. Biol. 111, 367 (2022)
2.?FOXM1 network in association with TREM1 suppression regulates NET formation in diabetic foot ulcers: A.P. Sawaya, et al.; EMBO Rep. 23, e54558 (2022)
3.?Multi-dimensional-double-spiral (MDDS) inertial microfluidic platform for sperm isolation directly from the raw semen sample: H. Jeon, et al.; Sci. Rep. 12, 4212 (2022)
4.?DYRK1A negatively regulates CDK5-SOX2 pathway and self-renewal of glioblastoma stem cells: B. Chen, et al.; Int. J. Mol. Sci. 22, 4011 (2021)
5.?Global phosphoproteomics reveals DYRK1A regulates CDK1 activity in glioblastoma cells: A. Recasens, et al.; Cell Death Discov. 7, 81 (2021)
6.?Hydrogen Peroxide Is Crucial for NLRP3 Inflammasome-Mediated IL-1β Production and Cell Death in Pneumococcal Infections of Bronchial Epithelial Cells; Journal of Innate Immunity: S. Surabhi, et al.; J. Innate Immun. 10, 1159 (2021)
7.?Licochalcone A inhibits hypoxia-inducible factor-1α accumulation by suppressing mitochondrial respiration in hypoxic cancer cells: M.K. Park, et al.; Biomed. Pharmacother. 133, 111082 (2021)
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NUCLEAR-ID??Blue DNA stain (GFP-CERTIFIED?)
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產(chǎn)品特點(diǎn)
●?藍(lán)色熒光特異性DNA染料
●?穩(wěn)定且純度高
●?可分析活細(xì)胞、可滲透細(xì)胞和固定細(xì)胞
●?無(wú)光漂白作用
●?無(wú)需RNase處理
●?與GFP和紅色染料兼容
●?經(jīng)驗(yàn)證適用于各種細(xì)胞密度
●?快速易用
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產(chǎn)品詳情：
產(chǎn)品名稱 | NUCLEAR-ID??Blue DNA stain (GFP-CERTIFIED?) |
產(chǎn)品貨號(hào) | ENZ-CHM103-0200 |
產(chǎn)品規(guī)格 | 200μl |
最大激發(fā)波長(zhǎng) | 350nm |
最大發(fā)射波長(zhǎng) | 461nm |
純度 | ≥95% (HPLC) |
外觀 | Frozen liquid |
應(yīng)用 | Flow Cytometry, Fluorescent detection |
短期儲(chǔ)存 | -20°C,?Protect from light. Avoid freeze/thaw cycles |
長(zhǎng)期儲(chǔ)存 | -80°C,?Protect from light. Avoid freeze/thaw cycles |
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