ENZO熱銷產(chǎn)品——ER-ID? Green assay kit內(nèi)質(zhì)網(wǎng)檢測試劑盒（綠色）
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Enzo Life Sciences公司的ER-ID??Green assay kit適用于活細胞或破膜醛固定細胞的染色。微摩爾級濃度的ER-ID?綠色染料足以對哺乳動物細胞進行染色，在人宮頸癌細胞系、T淋巴細胞系、Jurkat 細胞系、HeLa 細胞系以及骨肉瘤上皮細胞U2-OS中已經(jīng)得到了驗證。ER-ID??Green assay kit專門用于表達RFP或OFP的細胞系，以及表達藍色或青色熒光蛋白（BFP、CFP）的細胞。該試劑盒適用于活細胞或固定后細胞與探針（如標記抗體）或與Texas Red或香豆素類似光譜性質(zhì)的其他熒光綴合物結(jié)合使用。
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產(chǎn)品特點
● 新型內(nèi)質(zhì)網(wǎng)染料，可用于活細胞、破膜細胞或固定細胞的染色
●?與常見熒光染料和熒光蛋白兼容
●?高耐光漂白性、抗?jié)舛却銣绾凸廪D(zhuǎn)換
●?生產(chǎn)嚴格，以控制和消除非特異性結(jié)果
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實例分析
● ER-ID?綠色染色與含有KDEL序列的鈣網(wǎng)蛋白共定位，與橙色熒光蛋白（OFP）融合。
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●?用ER-ID?綠色染料（A）、Hoechst 33342染料（B）對HeLa活細胞進行染色，得到的合成圖像（C）。
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●?用4%多聚甲醛固定HepG2細胞20分鐘，并用ER-ID?標記30分鐘。下圖為標記3天后拍攝的照片。
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●?將HepG2細胞與ER-ID?孵育30分鐘，并用倒置顯微鏡（Zeiss）進行分析。
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產(chǎn)品詳情：
產(chǎn)品名稱 | ER-ID? Green assay kit |
產(chǎn)品貨號 | ENZ-51025-K500 |
產(chǎn)品規(guī)格 | 500 assays |
質(zhì)量控制 | A sample from each lot of ER-ID? Green assay kit is used to stain HeLa cells, using the procedures described in the user manual. The selectivity of the ER-ID? Green dye is evident. |
應(yīng)用 | Fluorescence microscopy Enzo Life Sciences’ ER-ID? Green assay kit contains a novel endoplasmic reticulum-selective dye suitable for live cell, or detergent-permeabilized aldehyde-fixed cell staining. |
長期儲存 | -20°C |
組分 | ER-ID? Green detection reagent: 50μl Hoechst 33342 nuclear stain: 50μl 10X assay buffer: 15ml |
產(chǎn)品說明 | The ER-ID? Green assay kit is a member of the CELLESTIAL? product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL? reagents |
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部分產(chǎn)品文獻引用
1.?Molecular interplay between NOX1 and autophagy in cadmium-induced prostate carcinogenesis: A. Tyagi, et al.; Free Radic. Biol. Med. 199, 44 (2023)?
2.?Ammonia induces amyloidogenesis in astrocytes by promoting amyloid precursor protein translocation into the endoplasmic reticulum: A. Komatsu, et al.; J. Biol. Chem. 298, 101933 (2022)?
3.?PLP1 mutations in patients with multiple sclerosis: identification of a new mutation and potential pathogenicity of the mutations: N.C. Cloake, et al.; J. Clin. Med. 7, 342 (2018), Application(s): Fluorescence microscopy?
4. Englerin A induces an acute inflammatory response and reveals lipid metabolism and ER stress as targetable vulnerabilities in renal cell carcinoma: A. Batova, et al.; PLoS One?12, e0172632 (2017),?Application(s):?Fluorescence microscopy
5. Cell growth on (“Janus”) density gradients of bifunctional zeolite L crystals: N.S. Kehr, et al.; ACS Appl. Mater. Interfaces?8, 35081 (2016)
6. The parathyroid hormone second receptor PTH2R and its ligand tuberoinfundibular peptide of 39 residues TIP39 regulate intracellular calcium and influence keratinocyte differentiation: E. Sato, et al.; J. Invest. Dermatol.?136, 1449 (2016)
7. Viral genome imaging of hepatitis C virus to probe heterogeneous viral infection and responses to antiviral therapies: V. Ramanan, et al.; Virology?494, 236 (2016),?Application(s):?Endoplasmic reticulum staining
8. Localization-Dependent Cell-Killing Effects of Protoporphyrin (PPIX)-Lipid Micelles and Liposomes in Photodynamic Therapy: S. Tachikawa, et al.; Bioorg. Med. Chem.?23, 7578 (2015),?Application(s):?Cell staining
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