高靈敏ELISA試劑盒,3小時(shí)內(nèi)可檢測低至1.40 pg/ml 6-酮前列腺素F1α
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6-酮-前列環(huán)素F1α(6-keto-PGF1α)是前列環(huán)素(PGI2)的穩(wěn)定水解產(chǎn)物。由于前列環(huán)素在緩沖液中的半衰期很短(2-3分鐘),通常通過測量尿液中的6-酮-前列環(huán)素F1α代謝物及其衍生物2,3-二酮-前列環(huán)素F1α來監(jiān)測前列環(huán)素的產(chǎn)生。前列環(huán)素具有自分泌功能,包括抑制血小板聚集、抑制加壓激素導(dǎo)致的血管收縮反應(yīng)、血小板解聚等特性以及生殖功能。這些效應(yīng)是由受體介導(dǎo)的cAMP的升高所引起的。前列腺素在動(dòng)脈粥樣硬化和保護(hù)心肌缺血方面有臨床應(yīng)用。
Enzo Life Sciences的6-酮-前列環(huán)素F1α比色法ELISA試劑盒,可在3小時(shí)內(nèi)得到檢測結(jié)果,在405nm處讀取OD值。該試劑盒特異性高,與結(jié)構(gòu)相關(guān)的分子交叉反應(yīng)性低。6-酮-前列環(huán)素F1α ELISA試劑盒已經(jīng)在各種類型樣品中得到了充分驗(yàn)證。欣博盛生物作為Enzo life sciences授權(quán)中國一級(jí)代理,可提供該產(chǎn)品,歡迎咨詢。
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產(chǎn)品特點(diǎn)
◆ 三小時(shí)內(nèi)可快速得到38份樣品(帶復(fù)孔)的實(shí)驗(yàn)結(jié)果
◆?高特異性,與結(jié)構(gòu)相關(guān)分子交叉反應(yīng)性低
◆ 已使用各種樣品進(jìn)行了充分驗(yàn)證,可靠性高
◆ 廣泛的文獻(xiàn)引用
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產(chǎn)品信息
產(chǎn)品貨號(hào) | ADI-900-004/ ADI-901-004 |
產(chǎn)品名稱 | 6-keto-PGF1α ELISA kit(6-酮前列腺素F1α ELISA試劑盒) |
規(guī)格 | 96?wells/ 5x96 wells |
其他名稱 | 6-keto Prostaglandin F1α |
靈敏度 | 1.40 pg/ml |
檢測范圍 | 3.2 - 50,000 pg/ml |
應(yīng)用 | ELISA, Colorimetric detection |
適用樣本類型 | 細(xì)胞培養(yǎng)上清,血清,唾液和尿液 |
檢測波長 | 405 nm |
適用種屬 | 不限種屬 |
運(yùn)輸 | Blue Ice Not Frozen |
長期保存 | +4°C |
試劑盒組分 | DxS IgG Microtiter plate, Conjugate, Antibody, Assay buffer, Wash buffer concentrate,? Standard, pNpp Substrate, Stop solution |
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標(biāo)準(zhǔn)曲線示例
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部分產(chǎn)品引用文獻(xiàn)
1. Laser Speckle Contrast Imaging-based diagnosis of severe mesenteric traction syndrome: Hemodynamics and prostacyclin - A prospective cohort study: A.A. Olsen, et al.; Microvasc. Res. 147, 104505 (2023)
2. Contribution of cyclooxygenase-1-dependent prostacyclin synthesis to bradykinin-induced dermal extravasation: M. Krybus, et al.; Biomed. Pharmacother. 148, 112786 (2022)
3. Differential effects of cyclo-oxygenase 1 and 2 inhibition on angiogenesis inhibitor-induced hypertension and kidney damage: K.M. Mirabito Colafella, et al.; Clin. Sci. (Lond.) 136, 675 (2022)
4. Estrogen modulates metabolic risk profile after resistance training in early postmenopausal women: a randomized controlled trial: T.V. Dam, et al.; Menopause 28, 1214 (2021)
5. Aspirin blocks formation of metastatic intravascular niches by inhibiting platelet-derived COX-1/thromboxane A2: S. Lucotti, et al.; J. Clin. Invest. 129, 1845 (2019)
6. Evidence for increased content of PGF2α, PGE2 and 6-keto-PGF1α in endometrial tissue cultures from heavy draft mares in anestrus with endometritis: M.J. Siemieniuch, et al.; J. Equine Vet. Sci. 77, 107 (2019)
7. Alterations in NO- and PGI2- dependent function in aorta in the orthotopic murine model of metastatic 4T1 breast cancer: relationship with pulmonary endothelial dysfunction and systemic inflammation: E. Buczek, et al.; BMC Cancer 18, 582 (2018)
8. Inhibitory Effects of an Orally Active Thromboxane A2 Receptor Antagonist, nstpbp5185, on Atherosclerosis in ApoE-Deficient Mice: S.W. Huang, et al.; Thromb. Haemost. 118, 401 (2018)
9. Advanced age in mares affects endometrial secretion of arachidonic acid metabolites during equine subclinical endometritis: M.J. Siemieniuch, et al.; Theriogenology 103, 191 (2017), Application(s): ELISA using culture supernatants
10. Colon cancer-derived myofibroblasts increase endothelial cell migration by glucocorticoid-sensitive secretion of a pro-migratory factor: Z. Drebert, et al.; Vascul. Pharmacol. 89, 19 (2017), Application(s): Determine the concentrations of prostanoids in conditioned medium from myofibroblasts and HUVECs, and in HUVEC lysates
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