蛋白質(zhì)羰基化是蛋白質(zhì)氧化損傷的一種,是氧化應激中一種不可逆的化學修飾。蛋白質(zhì)羰基化會引起蛋白質(zhì)結(jié)構(gòu)的改變,使其失去原有的生物學功能,最終導致細胞和組織功能紊亂。蛋白質(zhì)羰基化不僅是氧化損傷的一項重要指標,也參與多種疾病的生理和病理過程,比如衰老,凋亡,神經(jīng)退行性疾病等。蛋白質(zhì)樣品中蛋白質(zhì)羰基化的量可以用二硝基苯肼(DNP)衍生化并用比色法或免疫法檢測結(jié)合的DNP來測定。ELISA方法能夠用微克蛋白質(zhì)定量測檢測羰基化合物。
Enzo Life Sciences提供的Protein carbonyl ELISA kit是一種于抗體的檢測試劑盒。與比色分析相比,它具有更高的靈敏度、更低的背景、可檢測更多的樣本。
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產(chǎn)品特點
◆?高靈敏度ELISA,可檢測低水平的羰基化蛋白
◆?已在多種類型樣本中驗證
◆?超越半定量Western Blot分析的全定量檢測
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產(chǎn)品信息
產(chǎn)品貨號 | ALX-850-312-KI01 |
產(chǎn)品名稱 | Protein carbonyl ELISA kit |
規(guī)格 | 96 wells |
檢測時間 | 4.5 hours |
適用樣本類型 | 血漿,其他體液,細胞和組織提取物 |
適用種屬 | 不限種屬 |
檢測波長 | 450 nm |
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標準曲線示例
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部分產(chǎn)品文獻引用
1. Cardiovascular and Blood Oxidative Stress Responses to Exercise and Acute Woodsmoke Exposure in Recreationally Active Individuals: C.M. Williamson-Reisdorph, et al.; Wilderness Environ. Med. 33, 17-31 (2022)
2. Preliminary experience on the use of sucrosomial iron in hemodialysis: focus on safety, hemoglobin maintenance and oxidative stress: F. Reggiani, et al.; Int. Urol. Nephrol. 10, 11255 (2021)
3. A longitudinal study of neurotrophic, oxidative, and inflammatory markers in first-onset depression in midlife women: M.A. Pasquali, et al.; Eur. Arch. Psychiatry Clin. Neurosci. 268, 771 (2018), Application(s): ELISA using human serum
4. Antibiotic bedaquiline effectively targets growth, survival and tumor angiogenesis of lung cancer through suppressing energy metabolism: X. Wu, et al.; Biochem. Biophys. Res. Commun. 495, 267 (2018), Application(s): Human lung cancer cells
5. Antibiotic drug piperacillin induces neuron cell death through mitochondrial dysfunction and oxidative damage: S. Jiang, et al.; Can. J. Physiol. Pharmacol. 96, 562 (2018), Application(s): SH-SY5Y cell lysates
6. Plasma protein carbonylation in haemodialysed patients: focus on diabetes and gender: G. Colombo, et al.; Oxid. Med. Cell. Longev. 2018, 4149681 (2018), Application(s): ELISA using human plasma
7. The biological age linked to oxidative stress modifies breast cancer aggressiveness: M.D.M. Saez-Freire, et al.; Free Radic. Biol. Med. 120, 133 (2018), Application(s): ELISA using liver homogenates
8. Tigecycline as a dual inhibitor of retinoblastoma and angiogenesis via inducing mitochondrial dysfunctions and oxidative damage: Y. Xiong, et al.; Sci. Rep. 8, 11747 (2018)
9. Antibiotic tigecycline enhances cisplatin activity against human hepatocellular carcinoma through inducing mitochondrial dysfunction and oxidative damage: J. Tan, et al.; Biochem. Biophys. Res. Commun. 483, 17 (2017), Application(s): HCC cell lysates
10. Induction of Mitochondrial Dysfunction and Oxidative Damage by Antibiotic Drug Doxycycline Enhances the Responsiveness of Glioblastoma to Chemotherapy: Q. Tan, et al.; Med. Sci. Monit. 23, 4117 (2017), Application(s): Human glioblastoma cell lines A172 and U87 lysates
11. Targeting mitochondrial respiration selectively sensitizes pediatric acute lymphoblastic leukemia cell lines and patient samples to standard chemotherapy: X. Fu, et al.; Am. J. Cancer Res. 7, 2395 (2017)
12. Impact of chromium dinicocysteinate supplementation on inflammation, oxidative stress, and insulin resistance in type 2 diabetic subjects: an exploratory analysis of a randomized, double-blind, placebo-controlled study: Z.M. Saiyed, et al.; Food Nutr. Res. 60, 31762 (2016), Application(s): Measurement of biomarkers in plasma
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