自由基和其他活性種在許多心理和病理生理過程中發(fā)揮著重要作用。自由基一旦在細(xì)胞內(nèi)產(chǎn)生，就會(huì)破壞多種細(xì)胞成分，包括蛋白質(zhì)、脂質(zhì)和DNA。然而，在較低濃度下，自由基可以作為細(xì)胞信號(hào)傳導(dǎo)中的第二信使。
Enzo Life Sciences的ROS-ID??ROS/RNS detection kit可用熒光顯微鏡直接實(shí)時(shí)監(jiān)測(cè)活細(xì)胞中活性氧與活性氮（ROS/RNS）的產(chǎn)生。該試劑盒中包含三種檢測(cè)探針：NO檢測(cè)試劑（紅色，Ex/Em 650/670 nm），用于總ROS檢測(cè)的氧化應(yīng)激檢測(cè)試劑（綠色，Ex/Em 490/525 nm）和超氧化物檢測(cè)試劑（橙色，Ex/Em 550/620 nm）。試劑盒內(nèi)還包括陽(yáng)性對(duì)照，綠膿菌素和L-精氨酸，它們分別是ROS和NO產(chǎn)生的常見誘導(dǎo)劑；陰性對(duì)照N-乙酰-L-半胱氨酸和c-PTIO則分別是ROS和NO的常見清除劑。通過將三種檢測(cè)探針與一組特異性抑制劑、激活劑相結(jié)合，能夠區(qū)分超氧化物、一氧化氮和過氧亞硝酸鹽。
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產(chǎn)品特點(diǎn)
● 直接監(jiān)測(cè)活細(xì)胞中的活性氧和氮化物（ROS/RNS）
●?可區(qū)分超氧化物、一氧化氮和過氧亞硝酸鹽
●?高靈敏度、特異性和準(zhǔn)確性，可用于活細(xì)胞研究
●?與組織培養(yǎng)基的主要成分（酚紅、FBS和BSA）兼容
●?試劑盒內(nèi)包含整套試劑，包括ROS/RNS誘導(dǎo)劑和清除劑
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實(shí)驗(yàn)示例
圖1. 氧化應(yīng)激檢測(cè)試劑（綠色）、超氧化物檢測(cè)試劑（藍(lán)色）和NO檢測(cè)試劑（紅色）的激發(fā)和發(fā)射光譜。
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圖2. 使用ROS/RNS檢測(cè)試劑盒演示HeLa細(xì)胞中過氧亞硝酸鹽和NO生成的代表性實(shí)驗(yàn)。分析表明，由于NO與超氧化物的反應(yīng)，L-精氨酸和綠膿菌素的聯(lián)合處理產(chǎn)生過氧亞硝酸鹽（綠色熒光），但很少產(chǎn)生NO（紅色熒光）。
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產(chǎn)品信息
產(chǎn)品貨號(hào) | ENZ-51001-200 |
產(chǎn)品名稱 | ROS-ID??ROS/RNS detection kit |
別名 | Reactive oxygen species / Reactive nitrogen species |
規(guī)格 | 1*1Kit |
長(zhǎng)期保存 | -80°C |
試劑盒組分 | NO Detection Reagent (Red), 60μl Oxidative Stress Detection Reagent (Green), 300 nmoles Superoxide Detection Reagent (Orange), 300 nmoles NO Inducer (L-Arginine), 100 μl ROS Inducer (Pyocyanin), 1 μmole NO Scavenger (c-PTIO), 400 nmoles ROS Inhibitor (N-acetyl-L-cysteine), 2x10 mg 10X Wash Buffer, 15 ml |
應(yīng)用 | Fluorescence microscopy |
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部分產(chǎn)品引用文獻(xiàn)
1.?Hypoxia-induced oxidative stress promotes therapy resistance via upregulation of heme oxygenase-1 in multiple myeloma: K. Abe, et al.; Cancer Med. (2023)
2.?Dihydroartemisinin-transferrin adducts enhance TRAIL-induced apoptosis in triple-negative breast cancer in a P53-independent and ROS-dependent manner: X. Zhou, et al.; Front. Oncol. 11, 789336 (2022)
3.?Intermittent exposure of cultured endothelial cells to physiologically relevant fructose concentrations has a profound impact on nitric oxide production and bioenergetics: M.L. Fiorello, et al.; PLoS One 17, e0267675 (2022)
4.?S1P (Sphingosine-1-Phosphate)-Induced Vasodilation in Human Resistance Arterioles During Health and Disease: B. Katunaric, et al.; Hypertension 79, 2250 (2022)
5.?Extra-mitochondrial citrate synthase initiates calcium oscillation and suppresses age-dependent sperm dysfunction: W. Kang, et al.; Lab. Invest. 100, 583 (2020)
6.?Liposome-encapsulated bacillus calmette-guérin cell wall skeleton enhances antitumor efficiency for bladder cancer in vitro and in vivo via induction of amp-activated protein kinase: Y.M. Whang, et al.; Cancers (Basel) 12, 3679 (2020)
7.?Mesenchymal stem cell conditioned medium ameliorates diabetic serum-induced insulin resistance in 3T3-L1 cells: A. Sanap, et al.; Chronic Dis. Transl. Med. 7, 47 (2020)
8.?Isoorientin derived from Gentiana veitchiorum Hemsl. flowers inhibits melanogenesis by down-regulating MITF-induced tyrosinase expression: Q. Wu, et al.; Phytomedicine 57, 129 (2019)
9.?Low intensity ultrasound reduces high glucose reduced nitric oxide generation in retinal pigment epithelial cells: M.B. Karmacharya, et al.; Ultrasound Med. Biol. 44, 647 (2018)
10.?HEK-293 secretome attenuates kainic acid neurotoxicity through insulin like growth factor-phosphatidylinositol-3-kinases pathway and by temporal regulation of antioxidant defense machineries: C. Venugopal, et al.; Neurotoxicology 69, 189 (2017)
11.?Selective enhancing effect of metal ions on mutagenicity: N. Fujii, et al.; Genes Environ. 38, 21 (2016)
12.?Cell-to-cell diffusion of glucose in the mammalian heart is disrupted by high glucose. Implications for the diabetic heart: W.C. De Mello ; Exp. Cell Res. 334, 239 (2015)
13.?Increased Proinflammatory Cytokine Production and Decreased Cholesterol Efflux Due to Downregulation of ABCG1 in Macrophages Exposed to Indoxyl Sulfate: K. Matsuo, et al. ; Toxins (Basel) 7, 3155 (2015)
14.?Regulation of gene expression by tobacco product preparations in cultured human dermal fibroblasts: G.E. Malpass, et al.; Toxicol. Appl. Pharmacol. 279, 211 (2014)
15.?Enterococcus faecalis infection causes inflammation, intracellular oxphos-independent ROS production, and DNA damage in human gastric cancer cells: J.A. Strickertsson, et al.; PLoS One 8, e63147 (2013)
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