InvivoGen 新品推薦：核酸轉(zhuǎn)染增強(qiáng)試劑——NATE?

產(chǎn)品介紹：

NATE?是InvivoGen設(shè)計(jì)的一種核酸轉(zhuǎn)染增強(qiáng)劑，可以提高難轉(zhuǎn)染細(xì)胞的瞬轉(zhuǎn)與穩(wěn)轉(zhuǎn)效率，尤其適合人的單核細(xì)胞與小鼠巨噬細(xì)胞等難轉(zhuǎn)染的細(xì)胞，如外周血單核細(xì)胞（THP-1）和小鼠單核巨噬細(xì)胞白血病細(xì)胞（RAW 264.7），且只需在平時(shí)加轉(zhuǎn)染試劑操作前30min加入NATE?即可。值得注意的是，NATE對(duì)細(xì)胞溫和，不會(huì)對(duì)細(xì)胞培養(yǎng)產(chǎn)生任何進(jìn)一步的毒性。

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在真核細(xì)胞轉(zhuǎn)染過(guò)程中，外源性核酸(如質(zhì)粒)的主要障礙是會(huì)被胞質(zhì)傳感器檢測(cè)，如cGAS/STING、AIM2炎性小體和LC3介導(dǎo)的自噬。這些防御信號(hào)級(jí)聯(lián)的激活常常會(huì)降低轉(zhuǎn)染率和細(xì)胞存活率，特別是在難以轉(zhuǎn)染的細(xì)胞(如免疫細(xì)胞)中會(huì)更明顯。當(dāng)使用NATE?時(shí)，這些核酸傳感通路將被抑制，從而在轉(zhuǎn)染過(guò)程中保護(hù)質(zhì)粒并促進(jìn)其表達(dá)。

產(chǎn)品特色：

● 與常用轉(zhuǎn)染試劑?(e.g. GeneXPlus, Lipofectamine? LTX, and jetPRIME?) 及物理方法兼容。

●?更高的轉(zhuǎn)染率，也適用于大質(zhì)粒?(> 10kb)。

●?在所有的轉(zhuǎn)染測(cè)試方案中都顯示對(duì)細(xì)胞溫和，沒(méi)有毒性。

產(chǎn)品信息：

應(yīng)用舉例：

Top?- Transient transfection of an ~3 kb GFP-expressing plasmid into THP-1 cells was performed using GeneXPlus both in the absence (left) and presence (right) of NATE?. After 48 hours, the transfected cells expressing GFP were visualized by fluorescence microscopy.?Bottom -?Transient transfections of an ~3 kb GFP-expressing plasmid (left) and an 8 kb GFP-expressing plasmid (right) into THP-1 cells was performed using commonly used transfection methods such as GeneXPlus, Lipofectamine? LTX, jetPRIME?, and nucleofection. This was performed both in the presence (green) and absence (yellow) of NATE?. After 48 hours, transfection efficiency (% GFP-expressing cells) was measured using flow cytometry. Data are presented as a fold change compared to transfection without NATE??

Top -?Transient transfection of an ~3 kb GFP-expressing plasmid into RAW 264.7 cells was performed using Lipofectamine? LTX both in the absence (left) and presence (right) of NATE?. After 48 hours, the transfected cells expressing GFP were visualized by fluorescence microscopy.?Bottom -?Transient transfection of an ~3 kb GFP-expressing plasmid (left) and an 8 kb GFP-expressing plasmid (right) into RAW 264.7 cells was performed using commonly used transfection methods including Lipofectamine? LTX, jetPRIME?, FuGENE?, and nucleofection. This was performed both in the presence (green) and absence (yellow) of NATE?. After 48 hours, transfection efficiency (% GFP-expressing cells) was measured using flow cytometry. Data are presented as fold change compared to transfection without NATE?.

Stable transfection of an ~10 kb SEAP?expressing plasmid into RAW 264.7 cells was performed using Lipofectamine? LTX both in the absence (left) and presence (right) of NATE?. After 10 days in selection with Blasticidin, the number of stable clones expressing SEAP?(blue wells) was visualized using QUANTI?Blue?, a SEAP detection reagent.

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