CleanCap??FLuc mRNA (5moU) - (L-7202)
螢火蟲熒光素酶mRNA (Trilink cat#L-7202)
產(chǎn)品描述
FLuc?mRNA表達(dá)一種熒光素酶蛋白，該蛋白最初是從螢火蟲體內(nèi)分離出來的。熒光素酶通常用于動物細(xì)胞培養(yǎng)中的基因表達(dá)和細(xì)胞活性檢測。在熒光素底物的存在下能夠發(fā)光。
該mRNA使用Trilink公司專利加帽法cleancap產(chǎn)生的天然的cap1帽結(jié)構(gòu)，cleancap的加帽效率較高。該mRNA具有PolyA尾巴，且經(jīng)過5-甲氧基尿苷修飾，針對哺乳動物系統(tǒng)進(jìn)行了優(yōu)化，和天然成熟的mRNA相似。
產(chǎn)品細(xì)節(jié)
產(chǎn)品貨號 | L-7202 |
純化方式 | Passes Agarose Gel Mobility |
長度 | 1929 nucleotides |
堿基組成 | Fully substituted with 5-Methoxy-U |
濃度 | 1.0 mg/mL |
溶液 | 1 mM Sodium Citrate pH 6.4 |
轉(zhuǎn)換因子 | 40 μg/OD260 |
推薦儲存條件 | At or below -40°C |
應(yīng)用 | Reporter Genes |
加帽結(jié)構(gòu) | AG Start, Cap 1, CleanCap |
其他名稱 | CleanCap?Firefly Luciferase mRNA (5-methoxyuridine) |
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參考文獻(xiàn)：
References
Zinger, A;Cvetkovic, C;Sushnitha, M;Naoi, T;Baudo, G;Anderson, M;Shetty, A;Basu, N;Covello, J;Tasciotti, E;Amit, M;Xie, T;Taraballi, F;Krencik, R; . Humanized Biomimetic Nanovesicles for Neuron Targeting
Herrera, M;Kim, J;Eygeris, Y;Jozic, A;Sahay, G; . Illuminating endosomal escape of polymorphic lipid nanoparticles that boost mRNA delivery
Duran-Mota, JA;Yani, JQ;Almquist, BD;Borr . Polyplex-Loaded Hydrogels for Local Gene Delivery to Human Dermal Fibroblasts
Jansig, E;Geissler, S;Rieckmann, V;Kuenemund, A;Hietel, B;Schenk, M;Wussow, S;Kreideweiss, P;Panzner, S;Reinsch, C;Cynis, H; . Viromers as carriers for mRNA-mediated expression of therapeutic molecules under inflammatory conditions
Lacroix, C;Humanes, A;Coiffier, C;Gigmes, D;Verrier, B;Trimaille, T; . Polylactide-Based Reactive Micelles as a Robust Platform for mRNA Delivery
Ryals, RC;Patel, S;Acosta, C;McKinney, M;Pennesi, ME;Sahay, G; . The effects of PEGylation on LNP based mRNA delivery to the eye
Kim, J;Jozic, A;Sahay, G; . Naturally Derived Membrane Lipids Impact Nanoparticle-Based Messenger RNA Delivery
Gambari, R;Gasparello, J;Fabbri, E;Borgatti, M;Tamanini, A;Finotti, A; . Peptide Nucleic Acids for MicroRNA Targeting
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